Quick and efficient PCR cloning of highly accurate DNA fragments

Amplification with a high-fidelity proofreading polymerase, such as Invitrogen™ Platinum™ SuperFi™ DNA Polymerase, leaves PCR products with blunt ends rather than 3′-A overhangs, due to the extensive 3′ to 5′ exonuclease activity. Therefore, the vectors supplied in the Invitrogen™ Zero Blunt™ TOPO™ PCR cloning kits contain blunt ends to offer more effective ligation. Choose from over 30 subcloning, sequencing, or long-fragment kits for 95% cloning efficiency in just 5 minutes.

  • Quick—avoid extra ligation steps and laborious searches for appropriate restriction enzymes
  • Efficient—the lethal ccdB gene on our Zero Blunt TOPO vectors acts as a positive-selection method so that only clones with an insert will grow as colonies on your plate
  • Flexible—available with or without competent cells, in multiple reaction sizes and formats to suit your application

Find the right high-fidelity blunt-end cloning solution for you:


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