Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with the trusted Invitrogen™ Platinum™ hot-start technology, designed for the highest success in PCR. Featuring >100x the fidelity of Taq polymerase, Platinum SuperFi Green PCR Master Mix, included in the TOPO XL-2 PCR Cloning Kit, is ideally suited for cloning, mutagenesis, and other applications benefiting from superior sequence accuracy, such as working with long DNA fragments.
Figure 5. Platinum SuperFi DNA Polymerase exhibits superior fidelity. Relative fidelity values of different DNA polymerases. Polymerase fidelity was measured by next-generation sequencing. The background level of experimental error was estimated from PCR-free library sequencing data. The polymerase fidelities were normalized to that of Taq polymerase.
Figure 6. Platinum SuperFi DNA Polymerase has higher specificity in amplifying larger targets than the Invitrogen™ Elongase™ Enzyme Mix. Targets of 1 kb, 3 kb, 7 kb, 10 kb, and 13 kb in length from lambda genomic DNA were amplified following the manufacturers’ standards for PCR cycling parameters. The Elongase Enzyme Mix is recommended for the TOPO XL PCR Cloning Kit, and Platinum SuperFi DNA Polymerase is provided with the TOPO XL-2 Complete PCR Cloning Kit.
Figure 7. Platinum SuperFi DNA Polymerase shows greater specificity for both human and lambda gDNA targets than Clontech’s high-fidelity (HiFi) polymerase. Amplification protocols were followed per manufacturers’ recommendations. From a 50 µL PCR reaction, 6.5 µL of the human gDNA targets and 4 µL of the lambda gDNA targets were loaded into a 1% agarose gel stained with EtBr.