QuantiGene RNA Assays for Gene Expression Profiling
QuantiGene RNA assays are hybridization-based assays that utilize a branched DNA technology for signal amplification for the direct quantitation of gene expression transcripts. They provide a more robust and accurate measurement compared to other gene expression quantitation methods. The QuantiGene Plex and QuantiGene Singleplex RNA assays are designed to quantitatively measure RNA directly from cell or tissue homogenates and even directly from blood without RNA purification or enzymatic manipulation, thereby avoiding inefficiencies and variability introduced by human error or errors inherent to these processes.
The QuantiGene Plex Assay utilizes xMAP Luminex beads for multiplexing of 3 to 80 RNA targets followed by branched DNA-based signal amplification. Choose from a variety of preassembled panels or custom design your own plex using our design tool. The assay is performed in a 96-well plate and signal is detected using a Luminex instrument.
Video: QuantiGene Plex – How It Works
Utilize QuantiGene Singleplex and Singleplex HT assay when you need to accurately measure a single transcript from lysed cells, tissue homogenates, prepared blood or FFPE samples. The Singleplex Assay is performed in a 96-well plate and signal is detected using a standard luminometer. The Singleplex HT Assay is performed in a higher-throughput 384-well plate.
Video: QuantiGene Singleplex Assay – How It Works
Comparison of QuantiGene Assay to qPCR
For Research Use Only. Not for use in diagnostic procedures.