Discover ways to increase your
qPCR productivity

Increase your qPCR productivity and get more from your gene expression analysis.

Watch the videos below to learn some best practices for real-time PCR assays, including how to label technical replicates, the best quencher molecule, the purpose of ROX™ dye in your assay, and how to measure PCR efficiency, baselines, and thresholds.

In addition, we’ve included some helpful hints for assay design to help improve your future experiments.


Labeling technical replicates
How to Label Technical Replicates in Life Technologies Real-Time PCR Software -- Ask TaqMan®: Ep. 1

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Real-time PCR assay design hints
Hints for Real-Time PCR (qPCR) Assay Design -- Ask TaqMan®: Ep. 2

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TaqMan® quencher options
Which Quencher Molecule is Best for TaqMan® Probes in Real-Time PCR? -- Ask TaqMan®: Ep. 3

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How to accurately measure PCR efficiency
How to Measure PCR Efficiency of an Assay -- Ask TaqMan®: Ep. 4

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Understanding baselines
Baselines in Real-Time PCR -- Ask TaqMan®: Ep. 5

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Understanding thresholds
Real-Time PCR Thresholds and Where to Place Them -- Ask TaqMan®: Ep. 6

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What is ROX?
The Purpose of ROX™ in Real-Time PCR -- Ask TaqMan®: Ep. 7

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