DNA Phosphoramidites

TheraPure DNA phosphoramidites were engineered specifically for oligonucleotide manufacturers requiring tightly controlled impurity profiles, strictly controlled processes with thorough documentation, and a rigorous process change control. With our in-house high-definition, advanced analytical capabilities including LC-MS, GC, HPLC31P NMR and 1H NMR, DNA and RNA phosphoramidites are ideal for meeting possible regulatory requirements. However, collaborative, customized specifications are always welcome for further discussion.

For more than 30 years, our OEM and custom custom capabilities in research, development, and manufacturing of nucleic acids continue to be key resources for our global partners at molecular diagnostic, pharmaceutical, and life science research companies.

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TheraPure DNA Phosphoramidite highlights

  • Raw materials and intermediates are always QC tested using analytical technologies (listed above) 
  • State-of-the-art purification methods utilized to obtain the highest amidite purity
  • Overall, high purity and tight critical impurity level control yields excellent batch-to-batch reproducibility

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dA(Bz) CEP

dC(Bz) CEP

dG(iBu) CEP

dT CEP

  dA(Bz) CEP dC(Bz) CEP dG(iBu) CEP dT CEP
Cat. No. 27-2030 27-2032 27-2034 27-2036
Formula C47H52N7O7P C46H52N5O8P C44H54N7O8P C40H49N4O8P
Molecular weight 857.95 g/mol 833.93 g/mol 839.92 g/mol 744.82 g/mol
CAS # 98796-53-3 102212-98-6 93183-15-4 98796-51-1
HPLC purity*

≥ 99%

31P NMR purity*

≥ 99%

Solution Clarity Free from undissolved particulate at 0.2M solution in ACN
Appearance White to pale yellow powder
Residual Solvent ≤ 3.0% total % by weight
Shipping/Storage condition Ambient / –20 °C
Water content ≤ 0.3%
Material Origin Non-animal source (no TSE/BSE risk)
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* For more specification details, contact us.

Traditional base protection methods are used to ensure quality and reliability for routine oligonucleotide synthesis.


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dA(Bz) CEP

dC(Bz) CEP

dG(iBu) CEP

dT CEP

  dA(Bz) CEP dC(Bz) CEP dG(iBu) CEP dT CEP
Cat. No. 27-1730 27-1732 27-1734 27-1736
Formula C47H52N7O7P C46H52N5O8P C44H54N7O8P C40H49N4O8P
Molecular weight 857.95 g/mol 833.93 g/mol 839.92 g/mol 744.82 g/mol
CAS # 98796-53-3 102212-98-6 93183-15-4 98796-51-1
HPLC purity ≥ 98.0%
31P NMR purity ≥ 98.0%
Water content (KF Method) ≤ 0.3%
Shipping / Storage  condition Ambient / –20 °C
Solution Clarity Free from undissolved particulate at 0.2M solution in ACN
Material Origin Non-animal source (no TSE/BSE risk)
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Fast Deprotect DMF-dG, iPrPAC-dG, PAC-dA, Ac-dC, and iBu-dC phosphoramidites are ideal for applications requiring mild deprotection to preserve the integrity of oligonucleotides containing sensitive dyes, labels, or other modifications, which may be unstable with prolonged exposure to strong alkaline conditions.  Fast Deprotect phosphoramidites permit the removal of base protecting groups under mild or accelerated conditions without the need for special reagents. 


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iPrPAC-dG CEP

PAC-dA CEP

Ac-dC CEP

DMF-dG CEP

  iPrPAC-dG CEP PAC-dA CEP Ac-dC CEP DMF-dG CEP IBU-dC CEP
Cat. No. (TheraPure) N/A N/A 27-2027 N/A
N/A
Cat. No. (standard) 27-1726 27-1723 29-1727 27-1737 27-1725
Formula C51H60N7O9P C48H54N7O8P C41H50N5O8P C43H53N8O7P C43H54N5O8P
Molecular weight 946.05 g/mol 887.96 g/mol 771.8 g/mol 824.9 g/mol 839.92g/mol
CAS # 150065-82-0 110543-74-3 154110-40-4 330628-04-1 110522-84-4
HPLC purity |(TheraPure)* ≥ 98.0% ≥ 98.0% ≥ 98% | (≥ 99%) ≥ 98.0% ≥ 98.0%
31P NMR purity | (TheraPure)* ≥ 98.0% ≥ 98.0% ≥ 98% | (≥ 99%) ≥ 98.0% ≥ 98.0%
Shipping / Storage
condition
Ambient / –20 °C
 Material Origin  Non-animal source (no TSE/BSE risk)
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* For more specification details, contact us.

Process and supply chain control

  • Processes are monitored from material procurement through final QC and product release, providing batch-to-batch reproducibility
  • A controlled raw material supply chain and established vendor partnerships ensure consistency and security of supply
  • Purity of each DNA phosphoramidite lot is assessed by HPLC and 31P NMR analyses
  • Rigorously controlled manufacturing processes minimize levels of reactive P(III) impurities which could be incorporated into the oligonucleotide and result in the accumulation of by-products

Custom services

  • Custom manufacturing services are available to assist in the development or scale-up of phosphoramidite compounds
  • Specialty phosphoramidites can be manufactured with base or sugar (2′, 3′ or 5′ positions) modifications

Flexible packaging

  • Common oligo synthesizer pack sizes are available for immediate delivery
  • Custom and bulk packaging are also available. Contact us to learn more.

 

Not finding what you are looking for? We also offer RNA phosphoramidites, specialty phosphoramidites, dye-labeled phosphoramidites, 2′-modifications, linker and spacer phosphoramidites, and succinates and CPG support synthesis supports. Contact us to learn more.

Resources

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