Invitrogen™ AccuPrime™ DNA polymerases utilize AccuPrime™ accessory proteins for improved PCR fidelity, yield, and specificity. AccuPrime™ technology is available in Taq, Pfx, GC-Rich, and SuperMix formulations.

Overview of AccuPrime Technology

The thermostable AccuPrime accessory proteins enhance specific primer–template hybridization during every cycle of PCR, preventing mispriming and improving PCR specificity and yield. In addition, AccuPrime enzymes are designed with Platinum™ antibodies that are specific to the DNA polymerase and inhibit its activity at room temperature, providing an automatic hot start.

How AccuPrime technology works
How AccuPrime technology works.

AccuPrime DNA polymerase products

  AccuPrime™ Taq DNA Polymerase, High Fidelity AccuPrime™ Pfx DNA Polymerase AccuPrime™ GC-Rich DNA Polymerase AccuPrime™ Taq DNA Polymerase System AccuPrime™ SuperMix I AccuPrime™ SuperMix II
DNA polymerase(s) Blend of proofreading enzyme from Pyrococcus species GB-D and Taq DNA Polymerase AccuPrime Pfx DNA Polymerase AccuPrime GC-Rich DNA Polymerase cloned from Pyrolobus fumarius AccuPrime Taq DNA Polymerase AccuPrime Taq DNA Polymerase AccuPrime Taq DNA Polymerase
Platinum hot-start technology ✓ ✓   ✓ ✓ ✓
Enhanced specificity with AccuPrime accessory proteins ✓ ✓ ✓ ✓ ✓ ✓
Fidelity (compared to Taq polymerase) 9x 26x 2x 2x 2x 2x
Resulting amplicon end 3′-A, mixed Blunt Blunt 3′-A 3′-A 3′-A
Formats available Stand-alone enzyme with two-buffer system. Buffer I is optimized for plasmids, cDNA, and λ DNA. Buffer II is optimized for gDNA. Stand-alone enzyme Stand-alone enzyme with two-buffer system. Buffer A is optimized for GC-rich gDNA targets. Buffer B is optimized for non–GC-rich gDNA, cDNA, plasmids, and λ DNA. Stand-alone enzyme with two buffer system. Buffer I is designed for small gDNA amplicons (≤200 bp), cDNA, and plasmids. Buffer II is designed for gDNA (200 bp–4 kb). SuperMix I SuperMix II
Applications Long PCR, multiplex PCR up to 15-plex Cloning, mutagenesis Amplification of difficult (GC-rich) templates Multiplex PCR, allele-specific amplifications Multiplex PCR, allele-specific amplifications Multiplex PCR, allele-specific amplifications
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