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SNP genotyping using 48-, 96-, and 384-well real-time PCR instruments

TaqMan SNP Genotyping Assays use TaqMan 5´ nuclease assay chemistry to amplify and detect specific SNP alleles in purified genomic DNA samples. Each predesigned TaqMan SNP Genotyping Assay in our massive collection includes two allele-specific TaqMan MGB probes containing distinct fluorescent dyes and a PCR primer pair to detect specific SNP targets. These TaqMan probe and primer sets (assays) uniquely align with the genome to provide unmatched specificity for the allele of interest.

Find assays    Learn more

SNP genotyping workflow (for human and animal samples)

We provide multiple solutions to isolate and purify DNA.

Kits for genomic DNA extraction                                               
Explore our broad range of kits for purifying genomic DNA from a variety of samples including tissue, cells, blood, serum, plants, forensic samples, and more.

DNA Extract All Reagents Kit                                                        
Obtain PCR-ready DNA from a wide variety of sample types, ranging from blood to buccal swabs to plant tissues in five minutes.

We offer a variety of TaqMan Genotyping Assays for a number of real-time PCR analysis applications.

TaqMan SNP Genotyping Assays     Find assay ›
The Predesigned TaqMan SNP Genotyping Assays collection includes millions of genome‑wide human assays, including common 1,000 Genome SNPs, HapMap SNPs, and coding SNP assays.

Custom TaqMan SNP Genotyping Assays     Design assay ›
Create custom assays by submitting confidential target sequences to our secure assay design pipeline.

TaqMan Drug Metabolism Assays     Find assay ›
A comprehensive collection of inventoried assays optimized for genotyping SNPs, insertions and deletions (indels), and multi‑nucleotide polymorphisms (MNPs) in drug metabolism-related genes.

Prepare a reaction plate using TaqMan Assays and TaqMan master mix, along with your DNA samples. No additional reagents are needed.

TaqPath ProAmp Master Mix
Delivers accurate genotyping results, even from samples containing PCR inhibitors. Includes ROX passive reference dye.

 

Amplify DNA and achieve allelic discrimination by running the PCR reaction on an Applied Biosystems real-time PCR system and/or thermal cycler. To better evaluate the accuracy of genotype calls, perform real-time time PCR and obtain genotyping data for every cycle. Or for higher throughput, run PCR on a thermal cycler and then perform an endpoint read on a real-time instrument.

Thermocycler instruments

ProFlex PCR System
The ProFlex PCR System’s interchangeable block formats allow you to maximize your throughput or run independent experiments concurrently.

SimpliAmp Thermal Cycler
The SimpliAmp Thermal Cycler is an easy-to use, compact, and accurate thermal cycler designed to fit every lab’s essential PCR workflow.

Real-time PCR instruments

Compare instruments ›

QuantStudio 3 Real-Time PCR System
Simple to use for all levels of experience

QuantStudio 5 Real-Time PCR System
Easy controls for optimized experiments

QuantStudio 6 Flex Real-Time PCR System
High flexibility and intuitive design

QuantStudio 7 Flex Real-Time PCR System
High versatility and intuitive design

QuantStudio 12k Flex Real-Time PCR System
Maximum throughput, flexibility, and scalability

 

Process and interpret qPCR data with intuitive software data analysis tools for a range of applications.

Applied Biosystems qPCR Analysis Modules     Learn more ›
Our cloud-based applications can bring together multiple data sets, with analysis speeds up to 10 times faster than our desktop software. The Genotyping app includes improved visuals and integrated traces of allelic discrimination plots to allow thorough QC of SNP assays to accurately reflect the true signals versus background noise.

TaqMan Genotyper Software
TaqMan Genotyper Software is for use with TaqMan SNP Genotyping Assays (Predesigned, Custom & DME) in combination with 48-, 96-, and 384-well microplates or OpenArray Genotyping Plates, and offers a state-of-the-art genotype-calling algorithm, an intuitive user interface, and enhanced multi-plate analysis features.

We provide multiple solutions to isolate and purify DNA.

Kits for genomic DNA extraction                                               
Explore our broad range of kits for purifying genomic DNA from a variety of samples including tissue, cells, blood, serum, plants, forensic samples, and more.

DNA Extract All Reagents Kit                                                        
Obtain PCR-ready DNA from a wide variety of sample types, ranging from blood to buccal swabs to plant tissues in five minutes.

We offer a variety of TaqMan Genotyping Assays for a number of real-time PCR analysis applications.

TaqMan SNP Genotyping Assays     Find assay ›
The Predesigned TaqMan SNP Genotyping Assays collection includes millions of genome‑wide human assays, including common 1,000 Genome SNPs, HapMap SNPs, and coding SNP assays.

Custom TaqMan SNP Genotyping Assays     Design assay ›
Create custom assays by submitting confidential target sequences to our secure assay design pipeline.

TaqMan Drug Metabolism Assays     Find assay ›
A comprehensive collection of inventoried assays optimized for genotyping SNPs, insertions and deletions (indels), and multi‑nucleotide polymorphisms (MNPs) in drug metabolism-related genes.

Prepare a reaction plate using TaqMan Assays and TaqMan master mix, along with your DNA samples. No additional reagents are needed.

TaqPath ProAmp Master Mix
Delivers accurate genotyping results, even from samples containing PCR inhibitors. Includes ROX passive reference dye.

 

Amplify DNA and achieve allelic discrimination by running the PCR reaction on an Applied Biosystems real-time PCR system and/or thermal cycler. To better evaluate the accuracy of genotype calls, perform real-time time PCR and obtain genotyping data for every cycle. Or for higher throughput, run PCR on a thermal cycler and then perform an endpoint read on a real-time instrument.

Thermocycler instruments

ProFlex PCR System
The ProFlex PCR System’s interchangeable block formats allow you to maximize your throughput or run independent experiments concurrently.

SimpliAmp Thermal Cycler
The SimpliAmp Thermal Cycler is an easy-to use, compact, and accurate thermal cycler designed to fit every lab’s essential PCR workflow.

Real-time PCR instruments

Compare instruments ›

QuantStudio 3 Real-Time PCR System
Simple to use for all levels of experience

QuantStudio 5 Real-Time PCR System
Easy controls for optimized experiments

QuantStudio 6 Flex Real-Time PCR System
High flexibility and intuitive design

QuantStudio 7 Flex Real-Time PCR System
High versatility and intuitive design

QuantStudio 12k Flex Real-Time PCR System
Maximum throughput, flexibility, and scalability

 

Process and interpret qPCR data with intuitive software data analysis tools for a range of applications.

Applied Biosystems qPCR Analysis Modules     Learn more ›
Our cloud-based applications can bring together multiple data sets, with analysis speeds up to 10 times faster than our desktop software. The Genotyping app includes improved visuals and integrated traces of allelic discrimination plots to allow thorough QC of SNP assays to accurately reflect the true signals versus background noise.

TaqMan Genotyper Software
TaqMan Genotyper Software is for use with TaqMan SNP Genotyping Assays (Predesigned, Custom & DME) in combination with 48-, 96-, and 384-well microplates or OpenArray Genotyping Plates, and offers a state-of-the-art genotype-calling algorithm, an intuitive user interface, and enhanced multi-plate analysis features.