MicroRNAs (miRNAs) are small, noncoding RNA molecules that direct posttranscriptional suppression of gene expression. They have been shown to act as key regulators in many basic biological processes such as development, cell proliferation, differentiation, and the cell cycle. Emerging evidence also implicates miRNAs in the pathogenesis of human diseases such as cancers, metabolic diseases, neurological disorders and infectious diseases.

Common miRNA research applications:

  • miRNA profiling—identify differentially expressed mature miRNAs using qPCR in application areas like oncology, metabolic diseases, and autoimmune diseases
  • miRNA quantitation—specific quantification of mature miRNAs using qPCR
  • miRNA validation—confirm miRNA profiling  results using qPCR
  • miRNA functional analysis—miRNA mimics and miRNA inhibitors for functional studies
  • Noncoding RNA (ncRNA) analysis—reliable detection and quantitation of non-coding human, mouse, or rat transcripts longer than 200 nucleotides.
  • Small RNA analysis—detect and measure any small RNA
  • pri-miRNA analysis—detect and measure primary miRNAs from human, mouse, or rat species

Applied Biosystems TaqMan MicroRNA Assays are innovative tools for miRNA research-from isolation through discovery, profiling, quantitation, validation and functional analysis. We have developed two methods for sensitive, precise quantification and profiling of miRNA using real-time PCR TaqMan probe-based analysis.

  • TaqMan Advanced miRNA Assays use ligation-based universal reverse transcription for an easy streamlined workflow.
  • TaqMan MicroRNA Assays — this “classic” version employs target-specific stem-loop reverse transcription primers for 3’ extended templates for highly sensitive detection.

Find assays  Learn more


Real-time PCR microRNA workflow:

Isolate RNA

 

Isolate RNA using methods that preserve RNA integrity and expression profiles

  • RNA isolation kits
    Innovative Ambion and Invitrogen RNA extraction products

  • mirVana miRNA Isolation kits
    These kits use a rapid procedure to isolate total RNA, including small RNAs such as miRNA, small interfering RNA (siRNA), and small nuclear RNA (snRNA), from tissues and cells.  We can address a small number of targets or handle samples that require high throughput.

  • TaqMan miRNA ABC Purification Kit
    For use with the classic TaqMan MicroRNA assays, obtain high quality miRNA for sensitive and specific miRNA expression analysis from difficult sample types including blood, serum, plasma, FFPE, solid tissues, cultured cells, saliva, or urine

Design and optimize primers

Not required for Applied Biosystems TaqMan Assays. These assays have passed our industry leading bioinformatics design criteria.

Reverse Transcription

Convert your RNA into cDNA, over a wide range of RNA concentrations.

TaqMan Advanced miRNA cDNA Synthesis Kit
Provides miR-Amp Universal Amplification with universal primers and master mix to increase the amount of cDNA for each target with accurate detection as few as 60 copies from as little as 1pg of total RNA or 2 μL of serum/plasma. Pre-Amplification Step for TaqMan Advanced miRNA Assays.

Note that a pre-amplification step is performed after the RT. The miR-Amp master mix and miR-Amp primer mix are included in the TaqMan Advanced miRNA cDNA Synthesis Kit.

TaqMan MicroRNA Reverse Transcription Kit
Classic TaqMan microRNA kit for use with the TaqMan MicroRNA Assays to convert miRNA to cDNA (miRNA specific RT).  Best for 1-10 targets. Not compatible with TaqMan Advanced miRNA products.

Megaplex RT Primers (For TAC and OpenArray)
Designed to streamline experimental workflows, Megaplex RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis on a TaqMan MicroRNA Array.

Megaplex PreAmp Primers
Pre-amplification and reverse transcription primer for use with the classic TaqMan MicroRNA assay (not for use with TaqMan Advanced MicroRNA products) when sensitivity is of the utmost importance, Megaplex PreAmp Primers significantly enhance the detection of miRNAs expressed at low levels, enabling the generation of a comprehensive expression profile using very little sample—as little as 1 ng of input total RNA.

Note: When used with - OpenArray, TAC with less than 350ng of RNA and optional for TaqMan Array Cards with greater than 350ng of RNA. For single tube assays you can run pre-amplification with the appropriate protocol.

TaqMan PreAmp Master Mix
Stretch as little as 1 ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan Gene Expression assays

Amplify cDNA

Amplify your cDNA using TaqMan Assays and Applied Biosystems TaqMan master mix. Once you add your cDNA, these products contain all of the enzymes, buffers, primers and probe necessary to perform TaqMan real-time PCR analysis.

Find assays  Learn more

Run qPCR

Instruments for real time PCR and digital PCR

Compare instruments ›

Analyze

Process and interpret qPCR data with intuitive software data analysis tools for a range of applications

Isolate RNA

 

Isolate RNA using methods that preserve RNA integrity and expression profiles

  • RNA isolation kits
    Innovative Ambion and Invitrogen RNA extraction products

  • mirVana miRNA Isolation kits
    These kits use a rapid procedure to isolate total RNA, including small RNAs such as miRNA, small interfering RNA (siRNA), and small nuclear RNA (snRNA), from tissues and cells.  We can address a small number of targets or handle samples that require high throughput.

  • TaqMan miRNA ABC Purification Kit
    For use with the classic TaqMan MicroRNA assays, obtain high quality miRNA for sensitive and specific miRNA expression analysis from difficult sample types including blood, serum, plasma, FFPE, solid tissues, cultured cells, saliva, or urine

Design and optimize primers

Not required for Applied Biosystems TaqMan Assays. These assays have passed our industry leading bioinformatics design criteria.

Reverse Transcription

Convert your RNA into cDNA, over a wide range of RNA concentrations.

TaqMan Advanced miRNA cDNA Synthesis Kit
Provides miR-Amp Universal Amplification with universal primers and master mix to increase the amount of cDNA for each target with accurate detection as few as 60 copies from as little as 1pg of total RNA or 2 μL of serum/plasma. Pre-Amplification Step for TaqMan Advanced miRNA Assays.

Note that a pre-amplification step is performed after the RT. The miR-Amp master mix and miR-Amp primer mix are included in the TaqMan Advanced miRNA cDNA Synthesis Kit.

TaqMan MicroRNA Reverse Transcription Kit
Classic TaqMan microRNA kit for use with the TaqMan MicroRNA Assays to convert miRNA to cDNA (miRNA specific RT).  Best for 1-10 targets. Not compatible with TaqMan Advanced miRNA products.

Megaplex RT Primers (For TAC and OpenArray)
Designed to streamline experimental workflows, Megaplex RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis on a TaqMan MicroRNA Array.

Megaplex PreAmp Primers
Pre-amplification and reverse transcription primer for use with the classic TaqMan MicroRNA assay (not for use with TaqMan Advanced MicroRNA products) when sensitivity is of the utmost importance, Megaplex PreAmp Primers significantly enhance the detection of miRNAs expressed at low levels, enabling the generation of a comprehensive expression profile using very little sample—as little as 1 ng of input total RNA.

Note: When used with - OpenArray, TAC with less than 350ng of RNA and optional for TaqMan Array Cards with greater than 350ng of RNA. For single tube assays you can run pre-amplification with the appropriate protocol.

TaqMan PreAmp Master Mix
Stretch as little as 1 ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan Gene Expression assays

Amplify cDNA

Amplify your cDNA using TaqMan Assays and Applied Biosystems TaqMan master mix. Once you add your cDNA, these products contain all of the enzymes, buffers, primers and probe necessary to perform TaqMan real-time PCR analysis.

Find assays  Learn more

Run qPCR

Instruments for real time PCR and digital PCR

Compare instruments ›

Analyze

Process and interpret qPCR data with intuitive software data analysis tools for a range of applications


Additional miRNA categories

We have more options for performing microRNA and ncRNA studies using real-time PCR.

  • TaqMan Non-coding RNA Assays
    For reliable detection and quantitation of non-coding human, mouse, or rat transcripts longer than 200 nucleotides

  • TaqMan Pri-miRNA Assays
    Detect and measure primary miRNAs from human, mouse, or rat species

  • TaqMan miRNA Flexible Content Panels
    An easy way to select and design a combination of assays to analyze a large set of genes in a pathway, disease, or biological process in one of three formats—TaqMan Array Plates, TaqMan Array Cards, or OpenArray Plates—and one of three species: human, mouse or rat.
  • Custom TaqMan Small RNA Assays
    Custom assays are ideal to quantitate your small RNA when a predesigned assay is unavailable—all you need is the sequence of interest.

  • miRNA Mimics & Inhibitors
    More than 50,000 miRNA mimics that mimic endogenous miRNAs and enable miRNA functional analysis by up-regulation of miRNA activity and Inhibitors designed to specifically bind to and inhibit endogenous miRNA molecules and enable miRNA functional analysis by down-regulation of miRNA activity.