TaqMan Advanced miRNA Assays

Applied Biosystems TaqMan Advanced miRNA Assays enable highly sensitive and specific quantification of mature miRNAs using qPCR. Together with the TaqMan Advanced miRNA cDNA Synthesis Kit, this solution is designed to provide a streamlined workflow that allows for exceptional detection of multiple miRNA targets from a single sample.

  • Universal reverse transcription (RT)—one RT step for all TaqMan Advanced miRNA Assays.
  • Sensitive— Detect targets with as few as 60 copies of input miRNA into the cDNA synthesis reaction.
  • Specific—detect only mature miRNA and distinguish related highly homologous miRNAs with gold-standard TaqMan probe specificity.
  • Small sample input—detect and quantify mature miRNA from as little as 1 pg of total RNA or 2 μL of purified plasma or serum.
  • Versatile—compatible with tissue and biofluids including serum and plasma.
  • Fast delivery—all pre-designed assays ship out in 1–2 business days


Already know your research target of interest?

Search for a pre-designed TaqMan Advanced miRNA Assay using the tool below:

Looking to design your own assay? Email specialty_oligos@thermofisher.com to request a design for a custom TaqMan Advanced miRNA Assay when a pre-designed assay is not offered.

Availbility & sizes: Inventoried assays are pre-packaged in "Small" scale and are ready to ship.

taqman-array-cards-plate

Now available: TaqMan Advanced miRNA assays in card, plate, and OpenArray format

Enable highly sensitive and specific quantification of mature miRNAs using qPCR in higher throughputs.

Search for pre-designed arrays ›
Design your own custom array ›


TaqMan miRNA Assay selection guide

Format

Recommended TaqMan assay/array

Individual qPCR Assays

New TaqMan Advanced miRNA Assays
Comprehensive collection of pre-designed TaqMan qPCR assays for human, mouse and rat species

All pre-designed assays are inventoried and ship out in 1-2 days

Search for pre-designed assays ›

96-well plate

TaqMan Advanced miRNA  96 well array plates
Comprehensive collection of pre-plated, pre-designed panels available in both fast and standard 96 well formats.

Search for pre-designed 96 well plates ›

384-well microfluidic TaqMan Array card

TaqMan Advanced miRNA array cards
Small reaction volumes and streamlined workflow enable profiling up to 380 miRNA targets from as little as 1 ng of total RNA

Search for predesigned 384-well arrays ›
Design your own custom 384-well array ›

OpenArray plate TaqMan Advanced miRNA OpenArray panels
Run three samples per QuantStudio 12K Flex OpenArray plate, translating up to 818 assays per sample (up to 758 unique assays)

Contact: QuantStudioFrontDesk@lifetech.com
Custom 384 well plates Contact: specialty_plates@thermofisher.com

Workflow

mirVana™ miRNA isolation kits

mirVana miRNA Isolation Kits

mirVana miRNA Isolation kits use a rapid procedure to isolate total RNA, including small RNAs such as miRNA, small interfering RNA (siRNA), and small nuclear RNA (snRNA), from tissues and cells.

TaqMan Advanced miRNA cDNA Synthesis kit

TaqMan Advanced miRNA cDNA Synthesis Kit

The TaqMan Advanced miRNA cDNA Synthesis Kit is used for preparation of cDNA templates from the entire pool of mature miRNAs in a sample.

TaqMan Advanced miRNA cDNA Synthesis Kit

miR-Amp Universal Amplification

miR-Amp uses universal primers and master mix to uniformly increase the amount of cDNA for each target. miR-Amp reagents are included in the TaqMan Advanced miRNA cDNA Synthesis Kit.

Applied Biosystems real-time PCR instruments

Applied Biosystems real-time PCR instruments

With Applied Biosystems real-time PCR platforms, you get true value with excellent performance, reliability, and world-class support. Designed for compatibility with Applied Biosystems TaqMan Assays and the flexibility of unlimited real-time chemistry choices, we make it easy for you to get started with your experiments.

mirVana™ miRNA isolation kits

mirVana miRNA Isolation Kits

mirVana miRNA Isolation kits use a rapid procedure to isolate total RNA, including small RNAs such as miRNA, small interfering RNA (siRNA), and small nuclear RNA (snRNA), from tissues and cells.

TaqMan Advanced miRNA cDNA Synthesis kit

TaqMan Advanced miRNA cDNA Synthesis Kit

The TaqMan Advanced miRNA cDNA Synthesis Kit is used for preparation of cDNA templates from the entire pool of mature miRNAs in a sample.

TaqMan Advanced miRNA cDNA Synthesis Kit

miR-Amp Universal Amplification

miR-Amp uses universal primers and master mix to uniformly increase the amount of cDNA for each target. miR-Amp reagents are included in the TaqMan Advanced miRNA cDNA Synthesis Kit.

Applied Biosystems real-time PCR instruments

Applied Biosystems real-time PCR instruments

With Applied Biosystems real-time PCR platforms, you get true value with excellent performance, reliability, and world-class support. Designed for compatibility with Applied Biosystems TaqMan Assays and the flexibility of unlimited real-time chemistry choices, we make it easy for you to get started with your experiments.


Additional information

Species TaqMan Advanced miRNA assays are available for human, mouse, and rat miRNAs. We expect to increase the number of miRNA assays in alignment with the Sanger miRBase Registry.
Assay Components

TaqMan Advanced miRNA assays are delivered in a single tube containing the specific pre-formulated TaqMan Assay (TaqMan MGB probe, and forward and reverse primers).

Note: TaqMan Advanced miRNA cDNA Synthesis Kit (Cat. No. A28007)  and TaqManFast Advanced Master Mix (Cat. No. 4444556) are recommended for use with TaqMan Advanced miRNA assays.

Available Sizes
Product Catalog no. Number of reactions
TaqMan Advanced miRNA assays A25576 250 qPCR reactions (20 μL)
Shipping and Storage

Shipped at ambient temperature.
Store at –15ºC to—25ºC.

White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability

TaqMan Assay documentation files can be downloaded at thermofisher.com/taqmanfiles

Product bulletin: TaqMan Advanced miRNA Assays
White paper: TaqMan Advanced miRNA Assays – superior performance
Your Innovative Research: Detection of miRNAs in nipple aspiration fluid
Application note: TaqMan Advanced miRNA Assays
User guide: TaqMan Advanced miRNA Assays
Quick reference: TaqMan Advanced miRNA Assays
 White Paper: A technical guide to identifying miRNA normalizers using TaqMan Advanced miRNA Assays
 Reference Guide: miRNA from serum and plasma samples
 Application Note: Simultaneous detection of miRNA and mRNA on TaqMan Array Cards using the TaqMan Advanced miRNA workflow

Product Categories

Related Applications

Data Analysis Tools

Species TaqMan Advanced miRNA assays are available for human, mouse, and rat miRNAs. We expect to increase the number of miRNA assays in alignment with the Sanger miRBase Registry.
Assay Components

TaqMan Advanced miRNA assays are delivered in a single tube containing the specific pre-formulated TaqMan Assay (TaqMan MGB probe, and forward and reverse primers).

Note: TaqMan Advanced miRNA cDNA Synthesis Kit (Cat. No. A28007)  and TaqManFast Advanced Master Mix (Cat. No. 4444556) are recommended for use with TaqMan Advanced miRNA assays.

Available Sizes
Product Catalog no. Number of reactions
TaqMan Advanced miRNA assays A25576 250 qPCR reactions (20 μL)
Shipping and Storage

Shipped at ambient temperature.
Store at –15ºC to—25ºC.

White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability

TaqMan Assay documentation files can be downloaded at thermofisher.com/taqmanfiles

Product bulletin: TaqMan Advanced miRNA Assays
White paper: TaqMan Advanced miRNA Assays – superior performance
Your Innovative Research: Detection of miRNAs in nipple aspiration fluid
Application note: TaqMan Advanced miRNA Assays
User guide: TaqMan Advanced miRNA Assays
Quick reference: TaqMan Advanced miRNA Assays
 White Paper: A technical guide to identifying miRNA normalizers using TaqMan Advanced miRNA Assays
 Reference Guide: miRNA from serum and plasma samples
 Application Note: Simultaneous detection of miRNA and mRNA on TaqMan Array Cards using the TaqMan Advanced miRNA workflow

Product Categories

Related Applications

Data Analysis Tools

Resources

 White paper: TaqMan Advanced miRNA Assays
 Product Bulletin: TaqMan Advanced miRNA Assays
Applied Biosystems qPCR Analysis Modules
Software for microRNA and non-codingRNA analysis
Download your TaqMan documentation files
MicroRNA and non-coding RNA applications for qPCR
Real-Time PCR Troubleshooting Tool

Support