Custom Applied Biosystems™ TaqMan™ SNP Genotyping Assays provide a highly flexible technology for detection of polymorphisms within any genome. TaqMan™ Assays have a simple workflow and provide the quickest way to generate genotyping data. They are ideal for genotyping applications including screening, association, candidate region, candidate gene, or fine-mapping studies.
High Quality Assay Design Pipeline
Create your own assays by submitting confidential target sequences to our secure assay design pipeline using the Custom TaqMan® Assay Design Tool. This pipeline has generated millions of assay designs by utilizing heuristic rules deduced from both manufacturing and assay performance data. These assays use TaqMan® minor groove-binding (MGB) probes for superior allelic discrimination, improved signal-to-noise ratios, and design flexibility. All human SNP genotyping assays are functionally tested on 20 unique DNA samples to ensure assay amplification and sample clustering. These custom assays are designed, synthesized, formulated, optimized, and quality control tested.
A Complete Genotyping Solution
In addition to TaqMan SNP Genotyping Assays, we offer Applied Biosystems™ TaqMan™ Genotyping Master Mix and superior real-time PCR instrument platforms for processing and analyzing all TaqMan SNP Genotyping Assays. Our data analysis software automatically determines sample genotypes and generates a cluster plot to help you visualize data across samples or populations.
Part numbers for TaqMan® SNP Genotyping Assays
Catalog # - Human | Catalog # - Non Human | Size | No. of 25 µL reactions (96-well) | No. of 10 µL reactions (96-fast) | No. of 5 µL reactions (384-well) |
---|---|---|---|---|---|
4331349 | 4332077 | Small; 40x | 300 | 750 | 1,500 |
4332072 | 4332075 | Medium; 40x | 1,000 | 2,500 | 5,000 |
4332073 | 4332076 | Large; 80x | 2,400 | 6,000 | 12,000 |
Discrimination |
5' nuclease assay to discriminate between 2 alleles of a specific SNP for use in genotyping studies. |
Assay Components |
Each assay contains:
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Delivery Times |
Approximate delivery times are 4–6 days in the US and 6–10 days in Europe. |
Expiration Date |
5 years from date of manufacture. |
Documentation |
Compact disk containing:
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Assay Information File |
Includes: sales order number, ship date, part number, product type, assay ID, lot number, assay mix concentration, primer & probe concentration, reporter dyes & quenchers, primer and probe oligonucleotide sequences, and other information. |
Tracking/Identification |
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Shipping and Storage Conditions | Assays ship at ambient temperature. Store at -15°C to -25°C upon arrival. |
Citations
- Progress toward an efficient panel of SNPs for ancestry inference
Forensic Sci Int Genet. 2014 May;10:23-32.
Kidd KK, Speed WC, Pakstis AJ, Furtado MR, Fang R, Madbouly A, Maiers M, Middha M, Friedlaender FR, Kidd JR.
- Ancestry informative marker sets for determining continental origin and admixture proportions in common populations in America.
Hum Mutat. 2009 Jan;30(1):69-78. doi: 10.1002/humu.20822.
Kosoy R, Nassir R, Tian C, White PA, Butler LM, Silva G, Kittles R, Alarcon-Riquelme ME, Gregersen PK, Belmont JW, De La Vega FM, Seldin MF.
- Validation of the performance of a comprehensive genotyping assay panel of single nucleotide polymorphisms in drug metabolism enzyme genes.
Hum Mutat. 2008 May;29(5):750-6. doi: 10.1002/humu.20703.
Welch RA, Lazaruk K, Haque KA, Hyland F, Xiao N, Wronka L, Burdett L, Chanock SJ, Ingber D, De La Vega FM, Yeager M.
- Whole genome amplification of DNA for genotyping pharmacogenetics candidate genes.
Front Pharmacol. 2012 Mar 30;3:54. doi: 10.3389/fphar.2012.00054. eCollection 2012.
Philips S, Rae JM, Oesterreich S, Hayes DF, Stearns V, Henry NL, Storniolo AM, Flockhart DA, Skaar TC.
- Serum based assay accurately detects single nucleotide polymorphisms of IL28B and SOCS3 in HIV/HCV co-infected subjects.
AIDS Res Hum Retroviruses. 2014 Jun 19. [Epub ahead of print]
Shaffer A, Hubbard J, Townsend K, Kottilil S, Polis M, Masur H, Kohli A.
- Analysis of chosen polymorphisms in FoxP3 gene in children and adolescents with autoimmune thyroid diseases.
Autoimmunity. 2014 May 1. [Epub ahead of print]
Bossowski A, Borysewicz-Sańczyk H, Wawrusiewicz-Kurylonek N, Zasim A, Szalecki M, Wikiera B, Barg E, Myśliwiec M, Kucharska A, Bossowska A, Goscik, Ziora K, Górska M, Ksrętowski A.
- Targeted SNP Genotyping Using the TaqMan® Assay
Disease Gene Identification
Methods in Molecular Biology Volume 700, 2011, pp 77-87
Dorit Schleinitz, Johanna K. DiStefano, Peter Kovacs
- New generation pharmacogenomic tools: a SNP linkage disequilibrium Map, validated SNP assay resource, and high-throughput instrumentation systemfor large-scale genetic studies.
Biotechniques. 2002 Jun;Suppl:48-50, 52, 54.
De La Vega FM1, Dailey D, Ziegle J, Williams J, Madden D, Gilbert DA.