Custom Applied Biosystems™ TaqMan™ Expression Assays are the custom version of our predesigned real-time PCR 5' nuclease TaqMan Assays for relative quantification of RNA expression. Simply provide us with your target sequence of interest, or submit primer/probe sequences of your own design, and soon you'll be ready to perform RNA expression studies on any coding or non-coding RNA in any organism.

How to Order

This product can be purchased by visiting AppliedBiosystems.com.  Direct ordering through the Life Technologies website will be available soon.

Comparison of Custom and Custom Plus TaqMan Gene Expression
Assay products

There are two custom assay options to meet your RNA research needs: Custom TaqMan Gene Expression Assays and Custom Plus Applied Biosystems™ TaqMan™ RNA Assays. Both allow you to submit a target sequence to our TaqMan Assay design pipeline, which uses your input sequence to design custom primer and probe sequences. See the key differences between the two options below:

Feature Custom Plus TaqMan RNA Assays Custom TaqMan Gene Expression Assays
Bioinformatic analysis performed on input target sequences  
In silico QC performed on assay sequences  
Specificity option for gene-level or transcript-level detection  
Contains TaqMan MGB probe and 2 unlabeled primers
Runs under universal thermal cycling conditions
Assay sequences provided  
Available for any species *Available for any species with genome information in our database
Automatic check for predesigned TaqMan Assays which may detect input sequence

Custom Plus TaqMan RNA Assays are designed using the full bioinformatic power of the TaqMan Assay design pipeline, which uses proprietary algorithms that have generated millions of assay designs and is regarded as the benchmark in the industry for qPCR design. If you do not need bioinformatics or QC checks performed on your sequences, then our standard Custom TaqMan Gene Expression Assays will meet your needs.

Easy ordering

Target sequence submission is easy using the Custom Applied Biosystems™ TaqMan™ Assay Design Tool (CADT). Simply select your bioinformatic analysis and assay specificity preferences, and enter your target sequence. Sequence submissions are completely confidential; we will not share your target sequences or assay sequences with any third parties.

If you do not have a pre-defined input sequence, the CADT will help you to search for sequences by keyword (gene symbol, accession number, etc.) or by genomic location in a broad range of the most popular and well-studied model species ranging from human and mouse to pig, dog, cow, Arabidopsis, and more.

Custom TaqMan Assay Design Tool

Ordering information
Sku Product Name Size
4331348 Custom TaqMan® Gene Expression Assay, SM FAM 360 reactions, 20x
4332078 Custom TaqMan® Gene Expression Assay, MED FAM 750 reactions, 20x
4332079 Custom TaqMan® Gene Expression Assay, LG FAM 2900 reactions, 60x
4441114 Custom Plus TaqMan® RNA Assay, SM FAM 360 reactions, 20x
4441117 Custom Plus TaqMan® RNA Assay, MED FAM 750 reactions, 20x
4441118 Custom Plus TaqMan® RNA Assay, LG FAM 2900 reactions, 60x
4448487 Custom TaqMan® Gene Expression Assay, SM VIC PL 360 reactions, 20x
4448508 Custom TaqMan® Gene Expression Assay, SM VIC 360 reactions, 20x
4448488 Custom TaqMan® Gene Expression Assay, MED VIC PL 750 reactions, 20x
4448509 CustomTaqMan® Gene Expression Assay, MED VIC 750 reactions, 20x
4448492 Custom TaqMan® Gene Expression Assay, LG VIC PL 2900 reactions, 60x
4448510 Custom TaqMan® Gene Expression Assay, LG VIC 2900 reactions, 60x
4448511 Custom Plus TaqMan RNA Assay SM VIC PL 360 reactions, 20x
4448514 Custom Plus TaqMan RNA Assay SM VIC 360 reactions, 20x
4448512 Custom Plus TaqMan RNA Assay MED VIC PL 750 reactions, 20x
4448515 Custom Plus TaqMan RNA Assay MED VIC 750 reactions, 20x
4448513 Custom Plus TaqMan RNA Assay LG VIC PL 2900 reactions, 60x
4448516 Custom Plus TaqMan RNA Assay LG VIC 2900 reactions, 60x
References
  1. Bergmann O et al. (2011) Identification of cardiomyocyte nuclei and assessment of ploidy for the analysis of cell turnover. Exp Cell Res 2, 188-194.
  2. Hevir N, Sinkovec J, Rizner TL (2011) Disturbed expression of phase I and phase II estrogen-metabolizing enzymes in endometrial cancer: lower levels of CYP1B1 and increased expression of S-COMT. Mol Cell Endocrinol 1, 158-167.
  3. Colazzo F et al. (2011) Extracellular matrix production by adipose-derived stem cells: implications for heart valve tissue engineering. Biomaterials 1, 119-127.
Product literature