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Introducing the QuantStudio 3 and 5 systems. The first cloud-enabled real-time PCR systems

QuantStudio 3 virtual demo

Default
USB ports enable you to copy experiment templates to the instrument prior to a run and copy run files to your desired location once a run is completed.
Instrument Software Demo
Connect instrument to the network using a Wi-Fi adapter. Access the instrument from the cloud and send data to the cloud.
Slide to rotate ›
X
I need simplicity The QuantStudio™ 3 Real-Time PCR System is intuitive and easy for any level of user
Instrument Software Demo

QuantStudio 5 virtual demo

Default
USB ports enable you to copy experiment templates to the instrument prior to a run and copy run files to your desired location once a run is completed.
Instrument Software Demo
Connect instrument to the network using a Wi-Fi adapter. Access the instrument from the cloud and send data to the cloud.
Slide to rotate ›
The QuantStudio™ 5 Real-Time PCR System offers high performance with new features, allowing you to take charge of how you create and manage your results.
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I need more control
Instrument Software Demo
The QuantStudio™ 5 Real-Time PCR System offers high performance with new features allowing you to take charge of how you create and manage your results.

Thermo Fisher Cloud

Thermo Fisher Cloud allows you to set up experiments at your desktop, monitor instruments remotely, and analyze sophisticated data sets in minutes.
See Thermo Fisher Cloud software in action!


VeriFlex block

Helps save time with efficient experiment runs

VeriFlex™ technology offers 3 independent temperature zones for PCR optimization, and fast thermal cycling enables you to get results in as little as
30 minutes.


Touch screen and software

Explore the interactive instrument touch screen. The touch screen software
allows you to download templates from your online account, edit experiment
templates, run experiments, and upload runs directly to your online account.
QuantStudio® 3 and 5 demo
James Patterson
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95.0
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James Patterson
Sean
test@gmail.com
....
027-007-0708

System specifications

 
Performance
Dynamic range 10 logs 10 logs
Sensitivity (resolution) 1.5-fold difference 1.5-fold difference
Sensitivity (# of copies) 1 copy 1 copy
Optical detection
Excitation (light source) Bright white LED Bright white LED
Filters/colors 4 filters coupled 96 wells: 6 filters decoupled for up to 21 filter combinations
384 wells: 5 filters coupled
Excitation range 450—600 nm 96 wells: 450—680 nm
384 wells: 450—650 nm
Detection range 500—640 nm 96 wells: 500—730 nm
384 wells: 500—700 nm
Data acquisition Whole-plate imaging Whole-plate imaging
System
Dimensions (W x D x H) 27 x 50 x 40 cm 27 x 50 x 40 cm
Weight (approx) 27 kg 27 kg
Reaction volume

96 wells:

  • 0.1 mL block: 10-30 μL 
  • 0.2 mL block: 10-100 μL

96 wells:

  • 0.1 mL block: 10-30 μL 
  • 0.2 mL block: 10-100 μL Fast 

384 wells:

  • 5 - 20 μL
Sample capacity (wells) 96-well 96- and 384-well fixed formats
Maximum ramp rate 6.5°C/sec 6.5°C/sec
Average sample ramp rate 3.66°C/sec 3.66°C/sec
Temperature uniformity 0.4°C 0.4°C
Run time Less than 30 minutes Less than 30 minutes
Veriflex Block 3 zones 6 zones (96-well format only)
Heating/cooling method Peltier Peltier
Calibration Factory-calibrated Factory-calibrated
On-board memory Yes Yes
Electrical approvals IEC
CE
IEC
CE
Interactivity and connectivity
Touch screen Interactive touch screen Interactive touch screen
Real-time application viewing Touch screen and online Touch screen and online
Connectivity Cloud via LAN or Wi-Fi Cloud via LAN or Wi-Fi
Data transfer USB
LAN
Wi-Fi
USB
LAN
Wi-Fi
External devices 2D barcode reader via USB connection 2D barcode reader via USB connection
Online ecosystem Thermo Fisher Cloud Thermo Fisher Cloud
Applications
Research areas Infectious disease research
Pathogen detection
Translocation analysis
Gene detection
miRNA expression
Viral load analysis
Stem cell research
Plant sciences and agricultural biotechnology
Oncology research
Inherited disease research
Epigenetics
Synthetic biology
Key applications Gene expression profiling
Copy number analysis
Methylation analysis
HRM
SNP genotyping
Mutation scanning
miRNA profiling
castPCR™ analysis
Protein ligation assays
DME genotyping
Multiplexing
Software
Desktop operating system Microsoft WindowsTM 7 OS Microsoft WindowsTM 7 OS
Cloud Design and Analysis Software OS-independent through web browser on PC or MacTM on Thermo Fisher Cloud OS-independent through web browser on PC or MacTM on Thermo Fisher Cloud
Single-plate analysis Absolute gene expression, relative gene expression
SNP genotyping
Presence/absence
Melt
HRM
Multi-plate analysis
Gene expression studies
SNP genotyping studies
Run programming options Preoptimized protocol templates or ability to customize
Programmable and manual pause
Locked workflows
Preoptimized protocol templates or ability to customize
Programmable and manual pause
Locked workflows
Multiplexing Up to 4 targets 96-well: up to 6 targets
384 well: up to 5 targets
Chinese language software Yes Yes
Compliance ISO 130845 ISO 130845
MIQE-compliant RDML export format Yes Yes
Assays Over 9 million predesigned TaqMan Assays for gene expression, genetic variation, gene regulation, and protein expression
Custom TaqMan® Assays also available
Reagents SYBRTM Green dye
TaqMan® probes
Capable of running other chemistries (table available online)
SYBRTM Green dye
TaqMan® probes
Capable of running other chemistries (table available online)
Run modes
Stand-alone
PC-connected
Connect to Thermo Fisher Cloud via LAN or Wi-FI

Resources

Support