MicroRNAs are single-stranded, 19- to 25-nucleotide RNAs that regulate gene expression post-transcriptionally by binding to the 3’ untranslated regions (UTRs) of target mRNAs, inhibiting their translation. The high relative stability of miRNA in biofluids (e.g., plasma, serum, urine, saliva, etc.) and the ability of miRNA expression profiles to accurately classify discrete tissue types and disease states have positioned miRNA quantification as a promising new tool for a wide range of biomarker discoveries. MicroRNAs are gaining popularity in emerging applications such as early detection of cancer, cardiovascular diseases, and simultaneous detection of miRNA and mRNA from the same samples for pathway analysis, etc.

Featured resources
Video

Expression analysis of both mRNA and miRNA on the same TaqMan Array card

This webinar focuses on pancreatic cancer research using the simultaneous measurement and analysis of mRNA and miRNA in the same sample.

Videos
Introduction to MicroRNA

Get answers to popular qPCR questions with our library of quick and easy how-to videos covering a variety of topics including experiment setup, best practices, and guidance on products and technologies.

High correlation of miRNA quantitation data from matched FFPE and snap-frozen tissues using TaqMan MicroRNA Assays

Discusses the isolation and detection of miRNAs from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) samples and demonstrates the accurate and robust measurement of miRNA expression from comparable snap-frozen samples.

Real-Time PCR: Understanding Ct

Learn more about Ct (threshold cycle), the intersection between an amplification curve and a threshold line. It is a relative measure of the concentration of target in the PCR reaction.

TaqMan Advanced miRNA Assays—superior performance for miRNA detection and quantification

This whitepaper highlights the TaqMan probe based performance for detecting low-abundance miRNAs, measurement of small fold-changes in serum, and single-base discrimination of related miRNAs.

A technical guide to identifying miRNA normalizers using TaqMan Advanced miRNA Assays

Discussion of a workflow to identify the two or three most stable miRNAs in a set of samples and use them as endogenous controls to normalize miRNA expression in qPCR studies.

Webinars

Genetic Analysis for All webinars

Learn how genetic analysis techniques, including sequencing, NGS, and quantitative and digital PCR, are being used by researchers to advance knowledge and innovations in challenging disease and research questions.

Video

This video discusses the basic concepts of miRNAs and miRNA research.

Additional resources

Real-Time PCR Resource Library

Innovative tools and educational resources that help advance the work of researchers engaged in real-time PCR.