Your samples may not be mixed or centrifuged properly
- Rerun the assay, mixing the samples well (by pipetting up and down a few times) and performing the centrifugation steps as described in the protocol.
- Centrifuging the samples ensures that the contents of the wells are pooled at the bottom of each well, allowing for the most efficient PCR reaction and the most accurate endpoint read.
For Research Use Only. Not for use in diagnostic procedures.