The High Capacity cDNA Reverse Transcription Kit (formerly the High Capacity cDNA Archive Kit) delivers extremely high quality first-strand cDNA from total RNA. The kit was designed for synthesizing cDNA for short or long term archiving; it yields very quantitative reverse transcription products from 0.02 to 2 μg of input total RNA in a 20 μL reaction (up to 10 μg RNA for a 100 μL reaction). Downstream applications include real-time PCR, standard PCR, and labeling for microarray analysis.

For superior cDNA synthesis, check out Invitrogen SuperScript IV Reverse Transcriptase - available in a stand-alone enzyme, first-strand cDNA synthesis kit, master mix for qRT-PCR applications, and one-step RT-PCR system to suit all your research needs.

Benefits include:

  • Super-efficient–up to 100x higher cDNA yield
  • Super-sensitive–Ct values reduced by as many as 8 cycles for RT-qPCR
  • Super-robust–transcribes even from degraded or inhibitor-containing RNA samples
  • Super-fast–10 min reaction time”

 

Optimum performance in real-time PCR

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription (RT) kit designed for optimum performance in real-time PCR with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and others. The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template quantities. The High Capacity RNA-to-cDNA Kit formulation reduces RT time by at least half, requires few pipetting steps, and delivering high cDNA yields.

The High Capacity RNA-to-cDNA Kit consolidates the components of the RT reaction into two tubes; 2X RT buffer and 20X enzyme mix, including RNase inhibitor. These components have been optimized to provide superior cDNA archiving for real-time gene expression analysis, and a more streamlined workflow is achieved by minimizing the the number of reagent additions.