Peptides for Targeted Quantitation
Selected or multiple reaction monitoring (SRM/MRM) are methods of absolute quantitation (also termed AQUA) in targeted proteomics, whereby a complex sample is spiked with a stable isotope-labeled peptide that acts as internal standard. Heavy peptides for each target must be synthesized and evaluated for suitability. Low-cost crude peptides are generally adequate for targeted assay development. Peptide libraries and quality standards thus identified can then be synthesized at AQUA purity for SRM assays.
Targeted peptide features:
- Versatility—different peptide grades and turnaround times
- Accuracy—HeavyPeptide™ AQUA Custom peptides provide concentration precision for quantitation of proteins at very low concentrations.
- Quality—stable isotope standards (SIS) have >99% isotopic purity
- Flexibility—multiple formats are available in both heavy or standard amino acid options
- Compatibility—optimized for use with Thermo Scientific LC-MS platforms with data analysis fully supported by Pinpoint 1.4
Compare targeted peptide libraries
| PepoTec™ SRM Peptide Libraries | HeavyPeptide™ AQUA Basic | HeavyPeptide™ AQUA QuantPro | HeavyPeptide™ AQUA Ultimate | |
|---|---|---|---|---|
| Research stage | Discovery | Discovery, confirmation, or validation | Confirmation or validation | Validation or clinical |
| Grades | 3 | 1 | 1 | 1 |
| Standard (“light”) versions available | ✓ |
✓ |
✓ |
✓ |
| Description | Grade 1 (fast and easy), grade 2 (greater analysis), or grade 3 (maximum assurance) | AQUA Basic | AQUA QuantPro | AQUA Ultimate |
| Formulation | >0.1 mg in 0.1% TFA in 50% (v/v) acetonitrile/H2O | Lyophilized | 5 pmol/µL in 5% (v/v) acetonitrile /H2O |
5 pmol/µL in 5% (v/v) acetonitrile /H2O |
| Concentration Specification (using AAA) | NA | NA | ±10–25% | ±5–10% |
| Purity | Crude | >95% | >97% | >97% |
| QC | MS check or MS analysis | MS & analytical HPLC, AAA | MS & analytical HPLC, AAA | MS & analytical HPLC, AAA |
| Estimated Delivery Time | 2–3 weeks | 3–6-weeks | 3–6-weeks | 3–6-weeks |
Resources
For Research Use Only. Not for use in diagnostic procedures.