ProQuantum High-Sensitivity Immunoassays

A powerful innovation in protein quantitation

Invitrogen ProQuantum high-sensitivity immunoassays—a new platform innovation that provides researchers with an easy-to-run, high-performance assay and no proprietary instrument to purchase. By utilizing proximity ligation assay (PLA®) technology, we have combined the analyte specificity of high-affinity antibody–antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a highly-sensitive protein quantitation assay. With its ability to detect even lower levels of protein than traditional methods and very small sample consumption you can get the most out of your precious or limited samples. Invitrogen ProQuantum Immunoassay Kits are all greener products that generate less waste and have more sustainable packaging than traditional assays. To hear the technical details about this product innovation from an expert, watch the on-demand webinar. Click the red button below.

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The benefits of ProQuantum immunoassays include:

  • High-sensitivity – detect low levels of proteins with greater sensitivity than traditional methods (Figure 1)
  • Broad dynamic range – ≥5 logarithmic units, minimizing sample dilutions to ensure they fall within the range
  • Small sample consumption – use 2–5 μL of sample (instead of 75 μL for triplicate wells with other methods)
  • Fast, easy workflow – typically 2 hours from sample to answer, no wash steps, and a single 1-hour incubation
  • No proprietary instrument to purchase – runs on any real-time PCR instrument
  • Includes intuitive cloud-based software – comprehensive data analysis and statistical group–wise comparison

Here is how it works

ProQuantum immunoassays utilize a matched pair of target-specific antibodies each conjugated to a DNA oligonucleotide. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology. During antibody–analyte binding, the two DNA oligos are brought into close proximity, which then allows for ligation of the two strands and subsequent creation of template strand for amplification (Figure 2).

Figure 1. Standard curve for Invitrogen ProQuantum High-Sensitivity Human IFNγ, IL-1β, IL-8, and IL-6 immunoassay kits (red) shows larger dynamic range and improved separation at the low end of the curve for greater sensitivity than normal and ultrasensitive ELISAs (blue).

Figure 2. ProQuantum immunoassay schematic. (A) Antibody-conjugates binding to target (1-hr incubation). (B) Ligation and amplification of signal (in qPCR instrument).


Robust and intuitive Cloud-based analysis software

Use the software (Figure 3) to access customized plate and assay setup instructions as well as obtain results including robust statistical group–wise comparisons.

Figure 3. Intuitive software for easy and comprehensive data analysis.
 


Greener product and packaging

Thermo Fisher Scientific is committed to designing our products with the environment in mind—it’s part of how we enable our customers to make the world healthier, cleaner, and safer. This fact sheet provides the rationale behind the environmental claim that Invitrogen ProQuantum Immunoassay Kits generate less waste and have more sustainable packaging than traditional ELISA kits. To learn more download the green features fact sheet here.


Ordering ProQuantum immunoassays

Our introductory protein targets include:

To learn more about the technology and benefits of ProQuantum high-sensitivity immunoassays and how they can help you in your research, download the ProQuantum high-sensitivity immunoassays brochure featured below.

Learn more here or email us at invitrogenimmunoassays@thermofisher.com and we will connect you with a representative who can help.


Let us know what future targets you would like to see

We are continuously developing new ProQuantum immunoassays. You can contribute to our product development roadmap. Email us at invitrogenimmunoassays@thermofisher.com and let us know what protein targets you would like to see next.

Explore the entire immunoassay portfolio: thermofisher.com/immunoassays