Membranes for Western Blotting
PVDF (polyvinylidene difluoride) and nitrocellulose membranes are available in different pore sizes and dimensions for different application needs. A protein's properties (i.e., charge, hydrophobicity, etc.) affect its ability to bind to membrane surfaces, so finding the optimal membrane may require experimenting with your specific protein on different membranes. Below are some basic guidelines to help you choose the right membrane for your Western blotting needs.
Choosing your western blot membrane
Membrane type | Applications & uses* | Reprobe characteristics | Binding interaction |
---|---|---|---|
Nitrocellulose membranes | WB-low mol wt proteins (<20 kDa), S,N-nucleic acids (<300 bp), AA, DSB | Can be stripped & reprobed | Hydrophobic and electrostatic |
PVDF membranes (including Invitrolon™ PVDF) | WB, P, AA, SPA | Can be stripped & reprobed | Hydrophobic |
iBlot® Transfer Stack, Nitrocellulose Membrane |
WB-low mol wt proteins (<20 kDa) | Cannot be stripped & reprobed | Hydrophobic and electrostatic |
iBlot® Transfer Stack, PVDF Membrane |
WB | Can be stripped & reprobed | Hydrophobic |
Nylon | S, N, WB | Can be stripped & reprobed | Ionic, hydrophobic, and electrostatic |
Featured categories
Nitrocellulose Membranes
Sheets and rolls of nitrocellulose with binding properties suitable for western blot and other protein or nucleic acid methods.
PVDF Membranes
Sheets and rolls of high-quality polyvinylidene difluoride (PVDF) membrane that offer high protein and nucleic acid binding capacity.
Resources |
NEW | Protein Electrophoresis and Western Blotting Support Center Find tips, troubleshooting help, and resources for your protein expression applications. |
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For Research Use Only. Not for use in diagnostic procedures.