Proven technology with quick, 5-minute TOPO® cloning procedure

Our Bac-to-Bac® Baculovirus Expression Systems offer a quick, robust, and proven method to produce recombinant baculovirus, expressing your gene of interest in insect cells. Engineering the pFastBac™ vector with blunt TOPO® Cloning technology simplifies your cloning reaction to a 5-minute bench-top reaction compared to tedious and time-consuming restriction enzyme cloning, which can take 2–3 days of work.

Advantages of the Bac-to-Bac® Expression Systems:

  • High titers up to 108 pfu enable large-scale protein expression
  • pFastBac™ vector contains polyhedrin promoter for high yields of recombinant protein
  • Reliable, rapid 5-minute blunt TOPO® cloning saves time
  • N- and C-Terminal 6xHis tag for easy purification
  • TEV protease sites enable removal of N- or C-terminal tag yielding a native protein

Download the Bac-to-Bac® Expression Kit Handbook

Bac-to-Bac® method delivers high titers

Traditional baculovirus systems require purification and amplification of an initial low-titer viral supernatant. In contrast, the Bac-to-Bac® Baculovirus Expression System is faster and easier because the technology relies on generation of recombinant baculovirus by site-specific transposition in E. coli rather than homologous recombination in insect cells, which often results in low titers.

Our pFastBac™ TOPO® Cloning vector recombines with the parent bacmid in DH10Bac™ E. coli competent cells to form an expression bacmid, which is then used for transfection into insect cells. After the very first transfection of the bacmid, you will produce high titers of pure recombinant baculovirus particles, saving weeks of precious time and enabling large-scale protein expression experiments.

Multiple kit formats for your convenience

We offer two formats of our Bac-to-Bac® Baculovirus Expression Systems. The Expression system format in the most complete kit available; these kits include pFastBac™ TOPO® cloning vector, fast competent cells for cloning, specialized competent cells bacmid generation and our CellFectin® II Transfection reagent. If you simply need the pFastBac™ vector, we offer a Cloning kit format, which contain the pFastBac™ TOPO® vector and competent cells for cloning in the gene of interest.