Bolt Bis-Tris Plus Gels are optimized gels for western blot analysis. Some features include:
- Superior band quality and band volume—Invitrogen Bolt Bis-Tris Plus chemistry is designed to deliver sharp, straight bands with higher band volume.
- Better protein resolution—more protein bands are detected due to 10% greater resolving distance and optimized gradient format.
- Preserved protein integrity—the legendary neutral-pH formulation minimizes protein modifications.
- Superior lot-to-lot consistency—our best-in-class manufacturing gives you highly reproducible gel performance, with a CV of only 2% for Rf resolution values.
Greater sensitivity with Bolt Bis-Tris Plus gels. Total cell extracts from A431 cells were transferred to NC and PVDF membranes from a 4–12% Bolt Bis-Tris Plus gel, and 4–20% Tris-glycine precast gel using the iBlot 2 Gel Transfer Device. The cells were treated with 100 ng/mL of human epidermal growth factor (hEGF) to up-regulate expression of the phospho-EGF receptor. The protein loads of the cell extracts ranged from 20 μg to 1.2 μg of extract. The blots were processed on the iBind Western System using the iBind processing protocols with a 1:200 dilution of Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb (Cell Signaling Technology) and a 1:2,000 dilution of anti-mouse HRP secondary antibody (Jackson ImmunoResearch). Detection was performed with Novex ECL HRP Substrate.
More accurate protein molecular weight assignments
Bolt gels are precast SDS-PAGE gels designed to give optimal resolution, band quality, and sensitivity for all your western blot analyses. Bolt gels are 10% longer than competitors’ available gels, which results in higher resolving distance and therefore better band resolution. The pore size of the Bis-Tris Plus chemistry and the well-to-well spacing on Bolt gels offers well-resolved, straight western blot bands with optimal band quality as compared to Bio-Rad’s gels, which may present poorly-resolved, diffuse or streaky bands. Besides providing you with more accurate protein molecular weight assignments, Bolt gels offer overall better-looking, publication-quality western blots.
Preserving the integrity of your proteins
Unlike traditional tris-glycine gels, Bolt Bis-Tris Plus Gels are bis-tris HCl buffered (pH 6.4) and have an operating pH of about 7.0. This neutral pH paired with a unique, gentle sample preparation protocol means your protein samples always reside in mild, nonacidic conditions, preserving the integrity of the proteins and minimizing protein modifications. This helps to ensure highly sensitive, accurate western blot results every time. Bolt gels deliver best-in-class inter-lot and intra-lot reproducibility, with resolution (Rf) CV of only 2%, so you can have increased confidence in your gel performance every day.
Bolt gels have been shown to be less fragile than Bio-Rad’s gels. The western workflow requires significant gel handling, so we developed Bolt gels to be stronger than other commercially available gels in order to minimize gel tearing.
More sample per well
Our unique, innovative wedge-shaped wells enable up to two times the protein sample loads, so you get easy sample loading and no more spillover.
Bolt gels are designed for speed without a compromise in quality. With our 7-minute Invitrogen iBlot 2 western blot transfer process, now you can be western-ready in as little as 42 minutes without sacrificing quality.