Primary & Validation Screening

Identify targets and confirm hits with our comprehensive screening options 

Large-scale screens are often used to identify potential targets involved in biological pathways or for drug discovery. Thermo Fisher Scientific offers a vast array of screening and validation options. For primary screening, choose from our best-in-class family of siRNA and miRNA libraries or next-generation CRISPR libraries. For efficient transfection into most cell lines, utilize our leading Lipofectamine™ transfection reagents. For target validation, options include instruments and reagents for qPCR, western blotting, and sequencing. For phenotypic evaluation, we offer an extensive variety of cellular analysis assays and instrumentation.

functional genomics screening workflow

Primary & validation screening

siRNA libraries

Predefined and custom sets of premium-quality Invitrogen™ Silencer™ and Silencer™ Select siRNA Libraries, for performing RNAi experiments in human, mouse, and rat cell systems (minimum order of 20 siRNAs for a custom library)

miRNA libraries

  • 100% coverage of miRBase Sequence Database Version 20 
  • All distinct individual miRNA mimics and inhibitors covered, and available typically with fast turnaround
  • Available as whole human, mouse, and rat libraries or smaller custom libraries (minimum order of 20 miRNAs for a custom library)
  • Practical 96-well or 384-well plate format for high-throughput screening, with complete plate layout information

CRISPR libraries 

CRISPR libraries are emerging as the next-generation tool for high-throughput functional genomics screening. Thermo Fisher Scientific introduces arrayed CRISPR libraries for screening applications.

Transfection

Expertise in cellular and molecular biology is behind the Invitrogen™ product portfolio of DNA reagents and consumables, which include the most-cited and trusted transfection and selection systems available. Choose from our broad range of transfection technologies, including lipid transfection and chemical and physical methods such as electroporation.

Real Time PCR

We offer a wide range of products for gene expression analysis, such as TaqMan™ probe–based assays and SYBR™ Green dye–based master mixes.

Western blotting

Get tools to obtain superior western data with minimal time and effort. We offer reagents, kits, and equipment to facilitate every step of western blot detection.

To learn more about what Thermo Fisher Scientific has to offer for your functional genomics screening workflow, select from our Screening and In Vitro & In Vivo studies phases.

Resources

For Research Use Only. Not for use in diagnostic procedures.