PSC research requires careful attention to culture conditions. From KnockOut media to Essential 8 media and cell therapy research media, Gibco media deliver culture with confidence. Find the right solution for your stem cell culture needs.
Pluripotent Stem Cell (PSC) Culture Media
Which pluripotent stem cell culture medium is right for you?
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Product |
CTS Essential 8 Medium | |||||
Ideal for |
Feeder-based human and mouse PSC culture, reprogramming, gene editing, and differentiation |
Translational or clinical research applications |
Robust maintenance of PSC culture, difficult cell lines, and superior performance in single cell passaging and gene editing |
Consistent PSC culture and superior reprogramming |
Translational or clinical research applications | |
Defined |
Animal origin components (BSA) | Xeno-free; no animal derived components, human derived |
Animal origin components |
Xeno-free; no animal derived components, human derived |
Animal origin free (AOF) | |
Recommended cell types |
Mammalian PSCs |
Human PSCs |
Human PSCs |
Human PSCs | Human PSCs | |
Weekend-free feeding schedule |
No |
No |
Yes |
No | ||
Genome editing |
Fair |
Fair |
Best |
Fair |
Fair | |
Robustness |
Good |
Good |
Best |
Fair |
Fair | |
| Recommended matrix |
Gibco Mouse Embryonic Fibroblasts and Attachment Factor (for human and mouse) | Geltrex Matrix | ||||
Recommended level of dissociation and passaging reagent |
Clump |
Versene Solution | ||||
Small cluster |
N/A |
N/A |
StemPro Accutase Cell Dissociation Reagent, |
StemPro Accutase Cell Dissociation Reagent, optional RevitaCell Supplement added to medium during first 18-24 hours post-passage for improved recovery |
StemPro Accutase Cell Dissociation Reagent, optional RevitaCell Supplement added to medium during first 18-24 hours post-passage for improved recovery |
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Single cell |
TrypLE Select Enzyme (for mouse) |
TrypLE Select or Tryple Express Enzyme, RevitaCell Supplement helpful but not required during recovery if using rhLaminin-521 |
TrypLE Select or Tryple Express Enzyme with RevitaCell Supplement added to medium during first 18-24 hours post-passage |
CTS TrypLE Select Enzyme with RevitaCell Supplement added to medium during first 18-24 hours post-passage | ||
Unless otherwise indicated, all products are For Research Use Only. Not for use in diagnostic procedures
*For human ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal Law restricts this device to sale by or on the order of a physician.
**For Research Use or Noncommercial Manufacturing of Cell-Based Products for Clinical Research. CAUTION: Not for direct administration into humans or animals.