Verifying the quality of your pluripotent stem cells (PSCs) is critical to moving your research goals forward. We have a variety of cellular and molecular methods to help you completely and cost-effectively characterize your PSCs. From the PluriTest compatible PrimeView Global Gene Expression Profile Assays, which provides quick verification of pluripotency, to TaqMan hPSC Scorecard Panel, which confirms trilineage differentiation potential, we have the tools you need to characterize with lines with confidence.
PSC Characterization Tools
Cellular and molecular PSC characterization methods
|Alkaline Phosphatase Live Stain||Antibody staining||PluriTest-Compatible PrimeView Global Gene Expression Profile Assays||TaqMan hPSC Scorecard Panel||KaryoStat and KaryoStat HD Assays|
|Product description||Easy identification of pluripotency without compromising cell integrity||Specific and flexible identification of PSCs||Cost-effective global confirmation of pluripotency marker expression||Evaluates pluripotency and confirms trilineage differentiation potential||Alternative to g-banding karyotyping; offers whole-genome coverage for accurate detection of chromosomal abnormalities|
|How specific are the results?||Low (stains stem and progenitor cells)||Medium (stains human ES and iPS cells)||High (whole-transcriptome gene expresion profile)||High (profiles expression of both PSC markers and early germ layer factors)||High|
|Will the cells remain viable?||Yes||No ||No||No||No|
|How long before I see results?||Stain PSCs typically in 20 minutes or less||Stain PSCs typically in 90–120 minutes||2 days||6–8 hours||3–4 days|
|Are data analysis tools included?||No||No||Yes, free online PluriTest analysis tool||Yes, free cloud-based software||Yes, free downloadable Chromosome Analysis Suite (ChAS) software|
|Is a reference standard included?||No||No||Yes ||Yes ||No|
|Are EVOS FLoid Cell Imager protocols available?||Yes||Yes||No||N/A||No|
|Training and expertise required||Minimal||Minimal||Moderate||Moderate||Moderate|
|Unit size||500 μL vial sufficient for staining twelve 6 cm dishes||100 μg||30 arrays (one sample/array) or 1, 16-sample array plate||1, 384-well plate Kit (4 samples/plate) or 2, 96-well plates (1 sample/plate)||24 arrays (one sample/array)|
|1. Non-destructive when used with antibodies qualified for live staining.; 2. Reference standard is included in FREE software.|
PSC characterization services
With access to a complete portfolio of characterization tools and cutting-edge instruments our CellModel Services team can help.