Stem Cell Intel Issue 9
Each quarter, Stem Cell Intel will provide you with the latest product news, upcoming industry events, and easy access to technical tools such as publications, protocols, FAQs, and more. To receive Stem Cell Intel, please click the subscribe button below.
Stay on the leading edge of stem cell research
There’s still time to check out the 3rd annual 24 Hours of Stem Cells event. Scientific presentations, content, resources, and more are all available on demand now through March 3, 2016.
Access the virtual event on demand to:
If you registered for the live event, you can access the event on demand using your same login information. If you did not, you can register now.
Your favorite B-27 and N-2 products have been super-sized
You asked, we listened. Your favorite Gibco B-27 and N-2 products are now available as convenient larger pack sizes. This includes classic B-27 Supplement (100 mL), B-27 Supplement minus Vitamin A (100 mL), B-27 Supplement minus Insulin (100 mL), and N-2 Supplement (50 mL). Order today and save money on these larger sizes.
Essential 8 Flex Medium—the only true weekend-free medium
There has been a lot of noise about weekend-free pluripotent stem cell (PSC) culture. Unfortunately, there just has not been the data to back up those claims. Only Gibco Essential 8 Flex Medium provides the data needed to assure you that your PSCs have the proper level of FGF2 activity to leave them for 48 hours without impacting long-term pluripotency (Figure 1).
Figure 1. FGF2 activity over time in PSC culture medium. Unlike other feeder-free PSC culture media, Essential 8 Flex Medium has been optimized to extend the activity of unstable components such as FGF2. Extended activity allows for routine culture without the daily feeding.
PSC Cardiomyocyte Differentiation Kit transitions from prototype to catalog
The catalog version of the PSC Cardiomyocyte Differentiation Kit is now available. Previously A25042SA, the kit is now identified as A2921201. The catalog version is identical to the prototype in both formulation and packaging.
A product insert is available and includes new tips for performing range-finding studies and representative images of cell confluence.
Webinar: Pluripotent stem cell culture systems
This free, on-demand webinar identifies appropriate media, matrices, and passaging reagents for your stem cell workflow needs. It will specifically review feeder-dependent and feeder-free culture systems available at Thermo Fisher Scientific and the advantages and disadvantages of each. Finally, the webinar will provide helpful tips and tricks to assist in successful transition of feeder-dependent PSCs to feeder-free culture in Essential 8 Medium.
Publication: A qPCR ScoreCard quantifies the differentiation potential of human pluripotent stem cells
A recent publication by hPSC Scorecard inventor Dr. Alex Meissner and first author Dr. Alex Tsankov demonstrates the quantitative power of the hPSC Scorecard Assay relative to teratoma formation. The article, entitled “A qPCR ScoreCard quantifies the differentiation potential of human pluripotent stem cells,” was published online on October 26th, 2015 in Nature Biotechnology. The effects of feeder-based and feeder-free culture conditions as well as both spontaneous and directed differentiation methods on a cell line’s score were also explored.
Publication: Optimization of human mesenchymal stem cell manufacturing with StemPro MSC SFM XenoFree
With an interest to remove animal components from their culture systems, researchers recently compared the use of Gibco StemPro MSC SFM XenoFree (SFM/XF) against FBS or human platelet lysate (HPL )-supplemented media. They were able to demonstrate the following:
- The immunophenotype of resting and IFN-γ– primed BMMSC and AdMSC remained unaltered in all media.
- Both HPL and SFM/XF increased the proliferation of BMMSC and AdMSC.
- Resting BMMSC and AdMSC, expanded in FBS or SFM/XF, demonstrated potent immunosuppressive properties in both non-primed and IFN-γ primed conditions.
- HPL-expanded MSC exhibited diminished immunosuppressive properties.
- Strong evidence supported the use of the SFM/XF medium, in contrast to the HPL medium, for the expansion of MSC towards therapeutic applications.
For Research Use Only. Not for use in diagnostic procedures.