Direct detection or derivatized amino acid analysis — the choice is yours

Direct detection or derivatized amino acid analysis — the choice is yours

Amino acid analysis is performed to support protein or peptide structure studies, evaluate fragmentation strategies, or detect the presence of atypical amino acids. Use Thermo Scientific™ Dionex™ AminoPac™ IC columns to perform direct amino acid analysis with or without pre- or postcolumn derivatization.

Amino acid IC columns


Columns

Recommended Use

Applications

Dionex™ AminoPac™ PA10 IC Columns High-resolution separation of free amino acids using the AAA-Direct Amino Acid Analyzer. AAA-Direct separation of free amino acids; simultaneous detection of vitamins, amino sugars, as well as separation of a wide range of sugars, phosphorylated amino acids, and common oxidation products of sulfur-containing amino acids.
Dionex™ AminoPac™ PA1 IC Columns High-speed, specialized amino acid applications with OPA or ninhydrin postcolumn derivatization. Separation by anion-exchange chromatography of phosphorylated and other amino acids, including strongly acidic amino acids and acid-labile amino acids under basic pH conditions.

Why AAA-Direct?

Dionex AAA-Direct systems offer a highly sensitive, direct-detection amino acid analyzer that requires no sample derivatization. Using this method, underivatized, hydrolyzed protein sample is simply reconstituted in water and directly injected for analysis. Used with the Dionex AminoPac PA10 anion exchange column for separation on an ion chromatography system with pulsed amperometry (IPAD)—a type of electrochemical detection—the method provides complete separation and quantitation of all common amino acids.

How is the sample prepared?

Hydrolysis of protein or peptide samples is the first step in processing a sample for amino acid analysis. The hydrolyzing reagents are then removed (typically by evaporation) and the hydrolysate is reconstituted in water or other compatible solvent.

What are the benefits of direct amino acid analysis?

The Dionex AAA-Direct system eliminates the need for derivatization and provides complete separation and quantitation of all common amino acids. The ability to separate complex mixtures of amino acids from the direct hydrolysis of biological samples makes this system most suitable in the analysis of urine and blood samples for the detection of metabolic diseases and samples with uncommon or unnatural amino acids which require flexible, easily modified, high performance chromatography.

Simple The hydrolyzed protein sample can simply be reconstituted in water and directly injected for analysis.
Reliable Derivatized amino acid products may not be stable, depending on the derivatization method, and the reagents used can cause interferences during chromatography.
Universal Derivatization is not always suitable for methylated, halogenated, phosphorylated, or sulfonated amino acids; directly detects secondary amino acids easily.
Fast Little sample preparation is needed.
Safe No toxic reagents are used.

Why AAA-Direct?

Dionex AAA-Direct systems offer a highly sensitive, direct-detection amino acid analyzer that requires no sample derivatization. Using this method, underivatized, hydrolyzed protein sample is simply reconstituted in water and directly injected for analysis. Used with the Dionex AminoPac PA10 anion exchange column for separation on an ion chromatography system with pulsed amperometry (IPAD)—a type of electrochemical detection—the method provides complete separation and quantitation of all common amino acids.

How is the sample prepared?

Hydrolysis of protein or peptide samples is the first step in processing a sample for amino acid analysis. The hydrolyzing reagents are then removed (typically by evaporation) and the hydrolysate is reconstituted in water or other compatible solvent.

What are the benefits of direct amino acid analysis?

The Dionex AAA-Direct system eliminates the need for derivatization and provides complete separation and quantitation of all common amino acids. The ability to separate complex mixtures of amino acids from the direct hydrolysis of biological samples makes this system most suitable in the analysis of urine and blood samples for the detection of metabolic diseases and samples with uncommon or unnatural amino acids which require flexible, easily modified, high performance chromatography.

Simple The hydrolyzed protein sample can simply be reconstituted in water and directly injected for analysis.
Reliable Derivatized amino acid products may not be stable, depending on the derivatization method, and the reagents used can cause interferences during chromatography.
Universal Derivatization is not always suitable for methylated, halogenated, phosphorylated, or sulfonated amino acids; directly detects secondary amino acids easily.
Fast Little sample preparation is needed.
Safe No toxic reagents are used.