Measure Kinase Activity in Real Time

  • Easy protocol - just mix, then read fluorescence
  • Kinetic format - suitable for mechanistic studies
  • Study substrate inhibitors
  • Fast—get results in under an hour

Omnia® Kinase Assays utilize a fluorescence peptide substrate-based technology for the rapid, homogenous and sensitive detection of enzymatic activity. Results are measured under optimal kinetics, physiological (mM) ATP, and in real time without use of radioactive tracers or specialized equipment. Increases in kinase activity result in a corresponding increase in fluorescence. The kinetic format of the assay also corrects for false positives (compound auto fluorescence) and false negatives (quenching), minimizing interference. The assay can be used to select for both ATP-competitive and ATP non-competitive (allosteric) inhibitors.

Learn more about the Omnia® Kinase Principle >

Kinase (112 total) Best Kit
AKT1 ST1
AKT2 ST1
AKT3 ST1
AMPK ST23
ARK5 ST31
AURORA A ST16
AURORA B ST16
AURORA C ST16
BRSK1  
CAMK1D (CAMKIδ) ST23
CAMK2A (CAMKIIα) ST23
CAMK2D (CAMKIIδ) ST23
CDC42BPA (MRCK/DMPK) ST18
CDC42BPB (MRCK/DMPK) ST18
CDK1/CyclinB ST7
CDK1/CyclinE ST7
CDK2/CyclinA ST7
CDK2/CyclinE ST7
CDK4/CyclinD1  
CDK5/p25NCK ST7
CDK5/p35 ST7
CDK6/CyclinD1  
CHEK1 (CHK1) ST11
CHEK2 (CHK2) ST3
CLK2 ST15
CLK3 ST15
CSNK1A1 (CK1) ST24
CSNK1D (CKIδ) ST24
CSNK1E (CK1ε) ST24
CSNK1G1 (CK1γ1) ST24
CSNK1G2 (CK1γ2) ST24
CSNK1G3 (CK1γ3) ST24
CSNK2A1 (CK2α1) ST22
DAPK3 ST1
DCAMKL2  
DYRK1A  
GSK3α ST13
GSK3β (C-terminal His) ST13
IKKβ (IKK2) ST20
IKKε ST24
MAP3K10 (MLK2) ST6
MAP3K11 (MLK3) ST8
MAP3K9 (MLK1) ST8
MAP4K2 (GCK) ST25
MAP4K4 (HGK) ST32
MAP4K5 (KHS1) ST26
MAPK1 (ERK2) ST17
MAPK10 (JNK3) ST10
MAPK11 (p38β) ST9
MAPK12 (p38γ) ST7
MAPK13 (p38δ)  
MAPK14 (p38α)  
MAPK3 (ERK1) ST17
MAPK8 (JNK1) ST10
MAPKAP-K2 (MK2) ST3
MAPKAP-K3 ST3
MAPKAP-K5 (PRAK) ST3
MARK2 ST31
MINK1 ST8
MLK1 ST8
MLK3 ST8
NEK1  
NEK2  
NEK4  
NEK6 ST27
NEK9  
PAK1 ST5
PAK2 ST5
PAK3 ST5
PAK4 ST5
PAK6 ST5
PAK7 ST5
PASK ST6
PHKG1 ST3
PHKG2 ST3
PIM1 ST26
PIM2 ST14
PKA ST5
PKCα ST8
PKCβI ST8
PKCβII ST8
PKCδ ST8
PKCε ST8
PKCη ST8
PKCγ ST8
PKCι ST8
PKCθ ST8
PKCζ ST8
PKCμ ST8
PKN1 (PRK1) ST18
PRKCN (PKD3) ST8
PRKD2 ST8
PRKG1 ST5
PRKG2 ST5
PRKX ST5
ROCK1 ST5
ROCK2 ST5
RPS6KA1 (RSK1) ST6
RPS6KA2 (RSK3) ST6
RPS6KA3 (RSK2) ST6
RPS6KA5 (MSK1) ST1
RPS6KA6 (RSK4) ST6
RPS6KB1 (p70S6K) ST6
SGK (SGK1) ST1
SGK2 ST4
SGKL (SGK3) ST4
STK22B (TSSK2) ST31
STK22D (TSSK1)  
STK3 (MST2)  
STK4 (MST1)  
TAOK2 (TAO1)  
TBK1 (IKK-ε) ST12

 

Kinase (77 total) Best Kit
ABL1 Y6
ABL1 E255K Y6
ABL1 G250E Y6
ABL1 T315I Y6
ABL1 Y253F Y6
ABLl2 (ARG) Y6
ALK Y4
AXL Y11
BLK Y5
BMX Y5
BTK Y5
CSF-1R (FMS) Y9
EGFR (ErbB1) Y12
EGFR T790M Y12
EGFR L858R Y10
EGFR L861Q Y12
EGFR1 Y7
EPHA1 Y6
EPHA2 Y5
EPHA4 Y12
EPHA5  
EPHA8 Y12
EPHB1 Y6
EPHB2 Y6
EPHB3 Y6
EPHB4 Y5
FER Y5
FGFR1 Y12
FGFR2 Y11
FGFR3 Y11
FGFR3 K650E Y12
FGFR4 Y10
FGR Y5
FLT1 (VEGFR1) Y8
FLT3 CD Y5
FLT3 D835Y Y5
FLT4 (VEGFR3) Y8
FRK Y5
FYN Y5
HCK Y5
IGF-1R Y4
IR (Insulin Receptor) Y4
IRR (Insulin-related receptor) Y4
ITK Y6
JAK2 Y11
JAK2 JH1 JH2 Y12
JAK2 JH1 JH2 V617F Y12
JAK3 Y7
KDR (VEGFR2) Y8
KIT Y9
KIT T670I Y12
LCK Y5
LYN A Y5
LYN B Y5
MERTK (cMER) Y6
MET (HGFR) Y12
MET (HGFR) M1250T Y9
MST1R Y8
MUSK Y1
NTRK1 (TRKA) Y8
NTRK2 (TRKB) Y5
PDGFRA (PDGFRα) Y9
PDGFRA D824V Y9
PDGFRB (PDGFRβ) Y14
PTK2 (FAK) Y3
RET Y12
RET V804L Y8
RET Y791F Y11
ROS1 Y4
SRC Y2
SRCN2  
SRMS Y12
SYK Y7
TEK (tie2) Y8
TYRO3 (RSE) Y5
YES Y5
ZAP70 Y8
Kinase (112 total) Best Kit
AKT1 ST1
AKT2 ST1
AKT3 ST1
AMPK ST23
ARK5 ST31
AURORA A ST16
AURORA B ST16
AURORA C ST16
BRSK1  
CAMK1D (CAMKIδ) ST23
CAMK2A (CAMKIIα) ST23
CAMK2D (CAMKIIδ) ST23
CDC42BPA (MRCK/DMPK) ST18
CDC42BPB (MRCK/DMPK) ST18
CDK1/CyclinB ST7
CDK1/CyclinE ST7
CDK2/CyclinA ST7
CDK2/CyclinE ST7
CDK4/CyclinD1  
CDK5/p25NCK ST7
CDK5/p35 ST7
CDK6/CyclinD1  
CHEK1 (CHK1) ST11
CHEK2 (CHK2) ST3
CLK2 ST15
CLK3 ST15
CSNK1A1 (CK1) ST24
CSNK1D (CKIδ) ST24
CSNK1E (CK1ε) ST24
CSNK1G1 (CK1γ1) ST24
CSNK1G2 (CK1γ2) ST24
CSNK1G3 (CK1γ3) ST24
CSNK2A1 (CK2α1) ST22
DAPK3 ST1
DCAMKL2  
DYRK1A  
GSK3α ST13
GSK3β (C-terminal His) ST13
IKKβ (IKK2) ST20
IKKε ST24
MAP3K10 (MLK2) ST6
MAP3K11 (MLK3) ST8
MAP3K9 (MLK1) ST8
MAP4K2 (GCK) ST25
MAP4K4 (HGK) ST32
MAP4K5 (KHS1) ST26
MAPK1 (ERK2) ST17
MAPK10 (JNK3) ST10
MAPK11 (p38β) ST9
MAPK12 (p38γ) ST7
MAPK13 (p38δ)  
MAPK14 (p38α)  
MAPK3 (ERK1) ST17
MAPK8 (JNK1) ST10
MAPKAP-K2 (MK2) ST3
MAPKAP-K3 ST3
MAPKAP-K5 (PRAK) ST3
MARK2 ST31
MINK1 ST8
MLK1 ST8
MLK3 ST8
NEK1  
NEK2  
NEK4  
NEK6 ST27
NEK9  
PAK1 ST5
PAK2 ST5
PAK3 ST5
PAK4 ST5
PAK6 ST5
PAK7 ST5
PASK ST6
PHKG1 ST3
PHKG2 ST3
PIM1 ST26
PIM2 ST14
PKA ST5
PKCα ST8
PKCβI ST8
PKCβII ST8
PKCδ ST8
PKCε ST8
PKCη ST8
PKCγ ST8
PKCι ST8
PKCθ ST8
PKCζ ST8
PKCμ ST8
PKN1 (PRK1) ST18
PRKCN (PKD3) ST8
PRKD2 ST8
PRKG1 ST5
PRKG2 ST5
PRKX ST5
ROCK1 ST5
ROCK2 ST5
RPS6KA1 (RSK1) ST6
RPS6KA2 (RSK3) ST6
RPS6KA3 (RSK2) ST6
RPS6KA5 (MSK1) ST1
RPS6KA6 (RSK4) ST6
RPS6KB1 (p70S6K) ST6
SGK (SGK1) ST1
SGK2 ST4
SGKL (SGK3) ST4
STK22B (TSSK2) ST31
STK22D (TSSK1)  
STK3 (MST2)  
STK4 (MST1)  
TAOK2 (TAO1)  
TBK1 (IKK-ε) ST12

 

Kinase (77 total) Best Kit
ABL1 Y6
ABL1 E255K Y6
ABL1 G250E Y6
ABL1 T315I Y6
ABL1 Y253F Y6
ABLl2 (ARG) Y6
ALK Y4
AXL Y11
BLK Y5
BMX Y5
BTK Y5
CSF-1R (FMS) Y9
EGFR (ErbB1) Y12
EGFR T790M Y12
EGFR L858R Y10
EGFR L861Q Y12
EGFR1 Y7
EPHA1 Y6
EPHA2 Y5
EPHA4 Y12
EPHA5  
EPHA8 Y12
EPHB1 Y6
EPHB2 Y6
EPHB3 Y6
EPHB4 Y5
FER Y5
FGFR1 Y12
FGFR2 Y11
FGFR3 Y11
FGFR3 K650E Y12
FGFR4 Y10
FGR Y5
FLT1 (VEGFR1) Y8
FLT3 CD Y5
FLT3 D835Y Y5
FLT4 (VEGFR3) Y8
FRK Y5
FYN Y5
HCK Y5
IGF-1R Y4
IR (Insulin Receptor) Y4
IRR (Insulin-related receptor) Y4
ITK Y6
JAK2 Y11
JAK2 JH1 JH2 Y12
JAK2 JH1 JH2 V617F Y12
JAK3 Y7
KDR (VEGFR2) Y8
KIT Y9
KIT T670I Y12
LCK Y5
LYN A Y5
LYN B Y5
MERTK (cMER) Y6
MET (HGFR) Y12
MET (HGFR) M1250T Y9
MST1R Y8
MUSK Y1
NTRK1 (TRKA) Y8
NTRK2 (TRKB) Y5
PDGFRA (PDGFRα) Y9
PDGFRA D824V Y9
PDGFRB (PDGFRβ) Y14
PTK2 (FAK) Y3
RET Y12
RET V804L Y8
RET Y791F Y11
ROS1 Y4
SRC Y2
SRCN2  
SRMS Y12
SYK Y7
TEK (tie2) Y8
TYRO3 (RSE) Y5
YES Y5
ZAP70 Y8

Table 1. Ordering information for reagents

Reagents Cat. No.
Omnia® Ser/Thr Reaction Buffer KB001A
Omnia® Tyr reaction Buffer KB002A
Omnia® ATP Solution AS001A

Omnia® Custom and Bulk Substrate Services

Should you not find your substrate of interest, Invitrogen also offers custom services for the generation of Omnia® peptide substrates. Omnia® substrates can be successfully produced for a wide variety of kinases. We also can provide milligram quantities of lyophilized substrate for your large screening projects.

Please contact us at discoverysciences@invitrogen.com for more information about these services or to request a quotation.