Custom Fluorescent Antibody and Protein Labeling

• Amine labeling—the most common form of labeling; amine reactive dyes are regularly used to label antibodies and other proteins for use in immunochemistry and immunoassays
• Thiol labeling—this is another option for labeling your protein of interest, if free thiols are present. Depending on the protein, labeling via thiol group may be useful for site specific labeling.
• SiteClick™ labeling—labeling using click chemistry can introduce the label via sugars found associated with the Fc region of many antibodies
• Other labeling methods—we also perform labeling utilizing other reactive chemistries, such as labeling a carboxylate and aldehyde groups, or introduce a label using crosslinking chemistries

We can conjugate your antibody or protein to:

• Alexa Fluor® dyes—for superior fluorescence performance across and beyond the visible spectrum, these dyes can be attached to antibodies and proteins without significant self-quenching, leading to brighter conjugates
• Qdot® nanocrystals—ideal for experiments requiring long-term photo-stability or single-excitation, multicolor analysis, these fluorescent particles have specially modified surfaces that facilitate conjugation to your protein of interest
• pHrodo™ Red and pHrodo™ Green pH indicator dyes—these pH sensitive dyes are almost non-fluorescent at neutral pH, but fluoresce brightly in acidic environments
• Pacific Blue™, Pacific Green™ and Pacific Orange™ dyes—UV excitable dyes specifically created for use with the violet laser found on many flow cytometers
• R-PE, APC, and Alexa Fluor® dye tandems—traditional fluorescent labels for flow cytometry applications, along with R-PE and APC tandems featuring our Alexa Fluor® dyes
• Traditional fluorophores and labels—these include biotin, FITC, TRITC, and Texas Red® dye
• Enzyme labels—choose from among RP, alkaline phosphatase, and others

If you don’t see the label you are interested in, just ask—in most cases, we can accommodate your request.

• Custom Zenon® labeling—Zenon® labeling technology allows for rapid non-covalent antibody labeling. If you can’t find the Zenon® labeling reagent you need, let us make one for you, with our custom labeling services. Download the custom labeling services quote form, complete, and email to the address on the form.
• Biopolymer labeling—we can also label certain biopolymers, such as dextrans, with many of the labels listed in the above section.