Intercellular junctions are specialized regions on cell borders that allow connections between neighboring cells. Intercellular junctions can be organized into three functional groups:

  • Occluding junctions–seal epithelial cells together to prevent leakage of molecules from one epithelial sheet to the other
  • Anchoring junctions–mechanically link cells and their cytoskeletons to adjacent cells or to the extracellular matrix
  • Communicating junctions–mediate the passage of chemical and electrical signals between interacting cells

Cell adhesion through intercellular junctions is essential to the body. A change in or loss of cell adhesion affects cell structure, cellular functioning and communication, and the extracellular matrix, which can result in severe health problems and diseases. Intercellular junction marker antibodies detect proteins specific to intercellular junctions and can aid in the study of the structure and functions of intercellular junctions. Intercellular junction marker antibodies can also help elucidate the role(s) that a protein may play in a number of tasks that are centered in or influenced by intercellular junctions. Quality Invitrogen intercellular junction marker antibodies are available for a variety of research needs.

Intercellular junction marker antibody targets

  • Cadherin P
  • CDH1
  • CDH2
  • CDH3
  • CDH5
  • CLDN3
  • CLDN5
  • CLDN8
  • CLDN18
  • CTNNB1
  • DPP4
  • DSG3
  • GJA1
  • MTDH
  • N-Cadherin
  • P-Cadherin
  • PANX1
  • PECAM1
  • TJP1
  • TJP2
  • TJP3
  • TRPV4
  • Featured product data

    Flow cytometry analysis of N-cadherin on HeLa cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton X-100 for 20 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were labeled with N-cadherin mouse monoclonal antibody (Cat. No. 33-3900, red histogram) or with mouse isotype control (pink histogram) at 2–4 µg/million cells in 2.5% BSA. After incubation at room temperature for 2–3 hours, the cells were labeled with Alexa Fluor 488-conjugated rabbit anti-mouse secondary antibody (Cat. No. A-11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Applied Biosystems Attune NxT Flow Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.


    Immunofluorescence analysis of N-cadherin on 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with N-cadherin mouse monoclonal antibody (Cat. No. 33-3900) at 1–2 µg in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488-conjugated rabbit anti-mouse IgG secondary antibody (Cat. No. A-11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade Gold Antifade Mountant with DAPI (Cat. No. S36938). Panel c is a merged image showing cell junctional localization of N-cadherin. Panel d shows no primary antibody. The images were captured at 20x magnification.


    Annotated product references

    Cat. No. 700178 was used in immunohistochemistry to report on a case of an unusual primary gastric Merkel cell carcinoma. Human pathology (Jun 2014; 45: 1310) "Primary gastric Merkel cell carcinoma harboring DNA polyomavirus: first description of an unusual high-grade neuroendocrine carcinoma." Capella C,Marando A,Longhi E,Bernasconi B,Finzi G,Parravicini C,Sessa F,La Rosa S

    Cat. No. MA5-12356 was used in immunohistochemistry to study prognostic and mortality risk factors in stage IIA breast tumors. Clinics (São Paulo, Brazil) (Jun 2011; 66: 607) "Evaluation of prognostic factors in stage IIA breast tumors and their correlation with mortality risk." Carvalho ST,Stiepcich MM,Fregnani JH,Nonogaki S,Rocha R,Soares FA