Expanding options for violet laser-excitable antibody conjugates

Super Bright dyes vs. Brilliant Violet dyes for flow cytometry

  • Super Bright antibody conjugates have comparable brightness to Brilliant Violet™ conjugates 
  • Both can be utilized in flow cytometry applications, typically without a need to adjust standard workflows
  • Both dye families are violet laser–excitable polymer dyes
  • Both require use of a special buffer to reduce nonspecific dye-dye interactions when two or more polymer-dye antibody conjugates are used
     

Super Bright dye antibody conjugates selection guide

Dye Super Bright 436 Super Bright 600 Super Bright 645 Super Bright 702
Comparable to Brilliant Violet™ 421
Brilliant Violet™ 605
Brilliant Violet™ 650
Brilliant Violet™ 711
Brightness
  • Similar to Brilliant Violet™ 421
  • Brighter than eFluor 450 and Pacific blue antibody conjugates
  • Similar to Brilliant Violet™ 605
  • Brighter than Pacific Orange antibody conjugates
  • Similar and sometimes superior brightness to Brilliant Violet™ 650
  • Similar to Brilliant Violet™ 711
Excitation  violet (405 nm) violet (405 nm) violet (405 nm) violet (405 nm)
Emission  436 nm 600 nm 645 nm 702 nm
Suggested bandpass filter 450/50 610/20 660/20 710/50
More about the dye View the Super Bright 436 dye dashboard View the Super Bright 600 dye dashboard View the Super Bright 645 dye dashboard View the Super Bright 702 dye dashboard
Comparative data See data See data See data See data

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