The 15 minute size separation and recovery system for library construction

  • Size separate free adapters from ligated product 10X faster in 3 easy steps
  • Optimized for tight, consistent recovery of DNA fragments <1Kb in size
  • Purified DNA is compatible with library construction protocols of major generation 2 sequencing platforms
  • Use recovered DNA directly in cloning reactions

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Size select DNA in 3 easy steps

With E-Gel SizeSelect II gels, you can separate and recover DNA for library construction in 3 easy steps,

E-Gel SizeSelect II gels are double-comb, precast agarose gels with a twist. Load your sample into the top row and electrophorese until your band or desired size range enters the bottom row. Then, easily remove the size-selected DNA with a pipette. That’s it. No additional steps are required.

Use the Invitrogen E-Gel Power Snap System (G8300), a compact, self-contained device with a built-in power supply and blue-light transilluminator, to run and visualize E-Gel SizeSelect II agarose gels. Reverse-run functionality on the E-Gel Power Snap System (G8300) allows for capturing bands of interest even if you miss the bands when they first pass through the collection wells.

Have DNA ready for library construction 10x faster

The E-Gel SizeSelect gels allow users to separate and recover DNA for short read fragment library construction in just 15 minutes. That's 10 times faster than using conventional gels, and purification kits. E-Gel SizeSelect gels are offered in a 2% agarose concentration, and validated  for the recovery of fragments in the 150-200bp size range. Once the DNA is recovered, no additional purification step is required before proceeding to the next step in library construction. E-Gel SizeSelect system has been used successfully in these generation 2 library construction protocols:

  • ABI's SOLiD Fragment Library Construction
  • ABI's SOLiD Mate Pair Library Construction
  • Illumina's Genome Analyzer Fragment Library Construction
  • Illumina's Genome Analyzer Paired-end Library Construction

Size select DNA in 3 easy steps

With E-Gel SizeSelect II gels, you can separate and recover DNA for library construction in 3 easy steps,

E-Gel SizeSelect II gels are double-comb, precast agarose gels with a twist. Load your sample into the top row and electrophorese until your band or desired size range enters the bottom row. Then, easily remove the size-selected DNA with a pipette. That’s it. No additional steps are required.

Use the Invitrogen E-Gel Power Snap System (G8300), a compact, self-contained device with a built-in power supply and blue-light transilluminator, to run and visualize E-Gel SizeSelect II agarose gels. Reverse-run functionality on the E-Gel Power Snap System (G8300) allows for capturing bands of interest even if you miss the bands when they first pass through the collection wells.

Have DNA ready for library construction 10x faster

The E-Gel SizeSelect gels allow users to separate and recover DNA for short read fragment library construction in just 15 minutes. That's 10 times faster than using conventional gels, and purification kits. E-Gel SizeSelect gels are offered in a 2% agarose concentration, and validated  for the recovery of fragments in the 150-200bp size range. Once the DNA is recovered, no additional purification step is required before proceeding to the next step in library construction. E-Gel SizeSelect system has been used successfully in these generation 2 library construction protocols:

  • ABI's SOLiD Fragment Library Construction
  • ABI's SOLiD Mate Pair Library Construction
  • Illumina's Genome Analyzer Fragment Library Construction
  • Illumina's Genome Analyzer Paired-end Library Construction

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Resources

Have questions about the E-Gel system or new to molecular biology applications? Access literature, selection guides, webinars, and additional educational resources below for more information.

Literature

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Selection guide

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Nucleic Acid Purification and Analysis Support Center

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