Ambion® Millennium™ Markers are designed to provide accurate size determination of single-stranded RNA between 0.5 and 9 kb and can be used in any northern blot protocol. Millennium™ Markers are a mixture of 10 discrete RNA transcripts with easy-to-remember sizes: 0.5, 1, 1.5, 2, 2.5, 3, 4, 5, 6, and 9 kilobases. The markers may be stained with ethidium bromide either during or after electrophoresis.

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Available Ambion® Millenium™ Markers

Because of their quantity and spacing, Millennium™ Markers are ideal for constructing very accurate standard curves for mRNA size determination. They also provide a reference scale for easy verification and comparison of known bands.

Millennium™ Markers-Formamide
are the same high-quality RNA markers, supplied in formamide. This marker set shows increased stability and resistance to RNase compared to other large molecular weight RNA markers.

BrightStar® Biotinylated Millennium™ Markers
are a mixture of unlabeled and biotinylated Millennium™ Markers that can be stained using ethidium bromide and detected nonisotopically using the BrightStar® BioDetect™ Nonisotopic Detection Kit or any biotin detection system.

Figure 1. (Left Panel) Millennium™ Markers and total RNA samples were electrophoresed on a 1% denaturing agarose gel and stained with ethidium bromide. (Right Panel) Ambion® BrightStar® Biotinylated Millennium™ Markers (1 µg) were electrophoresed on a 1% denaturing agarose gel and transferred to Ambion® BrightStar®-Plus Positively Charged Nylon Membrane using NorthernMax® blotting protocols. The 10 marker bands were then visualized using the BrightStar® BioDetect™ Kit.