Learn about methods and technologies for preparing and using antibodies and recombinant proteins in protein analysis techniques. Understand how specific primary and secondary antibodies are produced, screened, purified, and modified to enable their use in a myriad of protein detection (identification and measurement) applications.

  • Primary antibodies
  • Secondary antibodies
  • Antibody production
  • Immunogen preparation
  • Antibody purification
  • Antibody labeling 
  • ELISA
  • Multiplex assays
  • Microarrays
  • Immunoassays
  • Immunoprecipitation
  • Western blotting
  • Technical handbooks
  • Protein methods articles
  • Product application notes
  • Support center resources
  • Protocols
  • Product selection guides

Antibodies and proteins features

New in 2017

The Antibodies Learning Center organizes education information about antibodies. Antibody Methods reviews the steps of antibody production and purification. Cell Signaling Pathways provides visual maps of protein-mediated cellular processes, together with links to antibody search results.

Selection guide

Considerations for choosing a secondary antibody, including host and target species, targeted reactivity, purification, cross-adsorption, multiplexing, antibody class and subclass, whole antibodies vs. fragments, conjugates, and biotin-binding proteins.


Antibodies and proteins learning resources

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Type Title
Handbook Antibody-Based Tools for Evaluating Tumor-Related Inflammation (2016)
Handbook Antibody-Based Tools for Neurodegenerative Disease Research (2015)
Handbook Antibodies for Stem Cell Research (2015)
Handbook Exploring Cancer Proliferative Signaling Pathways Handbook (2015)
Pathways Cell Signaling Pathways (visual maps and antibody target search)
Methods article Antibody Methods Library
Methods article Introduction to Immunoglobulins
Methods article Introduction to Antibody Production and Purification
Methods article Antibody Production (Immunogen Preparation)
Methods article Antibody Isotyping and Characterization Methods
Methods article Antibody Purification Methods
Methods article Antibody Fragmentation
Methods article Immunoglobulin Structure and Classes
Methods article Antibody Labeling and Immobilization Sites
Methods article Antibody IgG Binding Proteins
Methods article Protein Methods Library
Methods article An Overview of Detection Probes
Methods article Secondary Antibodies as Probes
Methods article Enzyme Probes
Methods article Avidin-Biotin Interaction
Methods article Fluorescent Probes
Methods article Peptide Design
Methods article Peptide Synthesis
Methods article Amino Acid Physical Properties
Selection guide Primary Antibodies (Selection Tool)
Selection guide Secondary Antibodies (Selection Tool)
Selection guide How to Choose and Select a Secondary Antibody
Selection guide Immunoassays (Selection Guide)
Selection guide Find ELISA Kits by Target (Selection Guide)
Application note Protein Biology Application Notes
Application note Antibody Application Notes
Application note Characterization of antibody specificity using immunoprecipitation and mass spectrometry (IP-MS antibody validation).
Application note A step-by-step guide to successful chromatin immunoprecipitation (ChIP) assays and antibody selection.
Application note ABfinity rabbit monoclonal antibodies white paper.
Application note Carrier protein activation and conjugation data for immunogen preparation. Experiments with KLH variants and other immunogens to validate solubility, maleimide activation consistency, peptide conjugation efficiency and immunogenicity.
Application note Using antibody-binding proteins for antibody purification. Choose a format based on the specific application and scale.

Protein Biology Resource Library

Browse protein biology learning center content by collection (resource type) — protein methods articles, product application notes, selection guides, webinars, technical handbooks, etc.