Seminar
Aug 26, 2018 - Aug 28, 2018
Örebro University | Örebro, Sweden
Nordic Metabolomics Conference 2018

Comprehensive workflows, integrated solutions

Visit our booth at the Nordic Metabolomics Conference and learn how our solutions can help you harness the power of metabolomics to gain in-depth biological insight, facilitate biomarker discovery and advance scientific research. Our solutions in the areas of separation, detection, and software enables broader and deeper analyses into the metabolome, so you can go beyond the edge of what you ever thought possible.

Scientific program

Oral presentation

Tuesday, Aug 28, 11.40-12.00

Improved metabolome coverage and increased confidence in unknown identification through novel automated acquisition strategy combining sequential injections and MSn
Anas Kamleh, Thermo Fisher Scientific

Metabolite identification is a current bottleneck in the broad implementation of metabolomics, hindering biological interpretation of results. The development of fast-scanning high resolution accurate mass spectrometers has increased the number of detected metabolites in biological samples. However, many metabolites remain unidentified because of sample complexity and limitations in data-dependent acquisition (DDA). Data-independent acquisition (DIA) approaches can provide fragmentation for all precursor ions simultaneously, but result in complicated fragmentation spectra, further convoluting the identification process. This presentation describes a data-informed workflow that maximizes the number of metabolites interrogated by MS/MS and MSn, while minimizing the acquisition of uninformative spectra. This innovative workflow was used to analyze human plasma resulting in high confidence identifications, deeper metabolome coverage and enhanced biological knowledge generation.

Poster presentation

High-throughput Comprehensive Coverage of Hydrophilic and Hydrophobic Metabolites in Beer Utilizing a Dual Separation/High Resolution Accurate Mass Spectrometry System
Anas Kamleh, Thermo Fisher Scientific

Untargeted metabolomics studies aim to detect, measure, and identify as many metabolites as possible in a sample without bias. However, the diversity in structural, chemical, and physical properties of small molecules, renders complete coverage of the metabolome of a given sample challenging. Diverse separation strategies have been developed to improve metabolome coverage, but unavoidably have introduced bias toward hydrophilic or hydrophobic metabolites. Here, we explored the integration of reverse phase (RP) and hydrophilic interaction chromatography (HILIC) to maximize metabolites detected in a single sample, with high reproducibility and increased throughput. This dual UHPLC separation system was coupled to a Thermo Scientific Orbitrap mass spectrometer and used for untargeted metabolomic analysis of beer samples, identifying compounds important in discriminating flavor and indicating spoilage.

Featured products

Stop by booth no. 6 to learn more about our solutions for metabolomics research.

Mass Spectrometry

Chromatography

Software

Additional resources

Scientific program

Oral presentation

Tuesday, Aug 28, 11.40-12.00

Improved metabolome coverage and increased confidence in unknown identification through novel automated acquisition strategy combining sequential injections and MSn
Anas Kamleh, Thermo Fisher Scientific

Metabolite identification is a current bottleneck in the broad implementation of metabolomics, hindering biological interpretation of results. The development of fast-scanning high resolution accurate mass spectrometers has increased the number of detected metabolites in biological samples. However, many metabolites remain unidentified because of sample complexity and limitations in data-dependent acquisition (DDA). Data-independent acquisition (DIA) approaches can provide fragmentation for all precursor ions simultaneously, but result in complicated fragmentation spectra, further convoluting the identification process. This presentation describes a data-informed workflow that maximizes the number of metabolites interrogated by MS/MS and MSn, while minimizing the acquisition of uninformative spectra. This innovative workflow was used to analyze human plasma resulting in high confidence identifications, deeper metabolome coverage and enhanced biological knowledge generation.

Poster presentation

High-throughput Comprehensive Coverage of Hydrophilic and Hydrophobic Metabolites in Beer Utilizing a Dual Separation/High Resolution Accurate Mass Spectrometry System
Anas Kamleh, Thermo Fisher Scientific

Untargeted metabolomics studies aim to detect, measure, and identify as many metabolites as possible in a sample without bias. However, the diversity in structural, chemical, and physical properties of small molecules, renders complete coverage of the metabolome of a given sample challenging. Diverse separation strategies have been developed to improve metabolome coverage, but unavoidably have introduced bias toward hydrophilic or hydrophobic metabolites. Here, we explored the integration of reverse phase (RP) and hydrophilic interaction chromatography (HILIC) to maximize metabolites detected in a single sample, with high reproducibility and increased throughput. This dual UHPLC separation system was coupled to a Thermo Scientific Orbitrap mass spectrometer and used for untargeted metabolomic analysis of beer samples, identifying compounds important in discriminating flavor and indicating spoilage.

Featured products

Stop by booth no. 6 to learn more about our solutions for metabolomics research.

Mass Spectrometry

Chromatography

Software

Additional resources

Register to receive copies of our presentation/poster after the conference