APEX™ Antibody Labeling Kits utilize a unique solid-phase labeling method that enables an efficient, convenient and reliable method for covalently attaching fluorescent labels to very small amounts of IgG antibody in the presence of any contaminants including stabilizing proteins and amine-containing buffers.

  • Reliability – Produces conjugates with stable, covalent attachment of superior fluorescent labels
  • Convenience – Requires only 10 –20 µg of IgG antibody per labeling reaction, regardless of any purity limitations
  • Complete - Includes everything required to perform 5 separate labelings

APEX™ Antibody Labeling Kits Products

Fluorophore Ex/Em† Cat. no.
Emission Color*
Flow Cytometry Imaging  
Alexa Fluor® 488 495/519 A10468              ●        ● Order
Alexa Fluor® 555 555/565 A10470          ● Order
Alexa Fluor® 568 578/603 A10494     Order
Alexa Fluor® 594 590/617 A10474          ● Order
Alexa Fluor® 647 650/665 A10475              ●        ● Order
Oregon Green® 488 496/524 A10476              ●        ● Order
Pacific Blue™ dye 410/455 A10478              ●        ● Order
Biotin-XX N/A A10495     Order
* Gray represents fluorescence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm.  

How APEX™ Antibody Labeling Kits Work

The APEX™ Antibody Labeling Kits use a solid-phase labeling technique that captures the IgG antibody on the resin inside the APEX™ antibody labeling tip (Figure 1). Any contaminants, including stabilizing proteins or amine-containing buffers, are simply eluted through the tip. After applying the amine-reactive label, a fluorescent IgG conjugate is ready for use in any imaging or flow cytometry application in as little as 2.5 hours with very little hands-on time.

Product Information Sheet


APEX™ Antibody Labeling Kits
Figure 1. APEX™ antibody labeling tip.
 
Each APEX™ Antibody Labeling Kit includes:
Material Component Amount
Reactive fluorescent label
A
5 vials
Antibody labeling tips
B
5 each
Tip wash buffer
C
1.8 mL
DMSO
D
100 μL
Labeling buffer
E
100 μL
Neutralization buffer
F
100 μL
Elution buffer
G
1 ml
Elution syringe
H
1

Need to Label Other Antibody Amounts?

Zenon® Kits

< 1-20 µg
Microscale Kits

< 1-20 µg
Antibody Labeling kits

< 1-20 µg



 

Protein Labeling Kits

< 1-20 µg

 

SAIVI™ Kits

< 1-20 µg

 

APEX™ Antibody Labeling kits

10-20 µg




Learn More about APEX™ Antibody Labeling Kits

Resources

BioProbes®

Read recently published information about APEX™ Antibody Labeling Kits in the BioProbes® 58 Journal

 

Antibody Labeling Brochure

No matter how much antibody you want to label, view all our Antibody Labeling Kits in this comprehensive brochure.