Isolate any biomolecule—
just add your biotinylated ligand

Invitrogen Dynabeads Streptavidin products are the gold standard for capturing, isolating, and handling biotinylated molecules. They are the most cited technology in many applications such as library prep for next-generation sequencing (NGS), DNA, RNA isolations, immunoassays.

High reproducibility, low background, high sensitivity, fast kinetics, and easy to automate make them attractive for a wide variety of applications.

Advantages of using Dynabeads Streptavidin products

  • High reproducibility
  • Low background binding
  • High sensitivity
  • Easily automated
  • No centrifugation, precipitation, or filtration—all you need is a magnet
  • Robust and gentle, with optimal binding kinetics
  • Few handling steps, no sample dilution, minimal sample loss

Absolute reproducibility

All Dynabeads products are produced with full control of parameters such as bead size, surface area, iron content, and magnetic mobility. The absence of excess physically adsorbed streptavidin helps ensure negligible leakage, and secures a minimal batch-to-batch variation. The uniform characteristics and unique reproducibility within (CV <3%) and between batches helps reduce costs associated with quality control testing (Figure 1). Whether for your research project or for IVD testing activities, you can rely on the consistent performance of Dynabeads products.

Fast and flexible

Dynabeads Streptavidin enables instant and efficient capture of biotinylated molecules via rapid liquid-phase kinetics. This increases speed and sensitivity compared to filters and plate-based approaches (Figure 2). Dynabeads products are truly spherical and have a large surface area per unit volume.  This enables a high and constant binding capacity.

Figure 1. Monosized superparamagnetic Dynabeads magnetic beads. Each bead has an even dispersion of magnetic material, encased within a thin polymer shell. This provides a specific and defined surface for binding your ligand. The true uniformity of all beads within each batch (typical CV <3%) provides consistent physical and chemical properties. Unique batch-to-batch reproducibility (typical CV <5%) enables reproducibility and quality of results.

Figure 2. Dynabeads Streptavidin binding kinetics are superior to traditional microtiter plates. The graph shows % binding of tumor necrosis factor (TNF) to immobilized antibody as a function of time. Courtesy of Dr. N-B Liabakk, Norwegian University of Science and Technology, Trondheim, Norway.

Robust and gentle

Dynabeads streptavidin products feature excellent mechanical and chemical stability. No iron leakage. No inhibition of enzyme activity. The monolayer of recombinant streptavidin helps ensure that the vast majority of biotin-binding sites are left sterically available for binding. The technology is also extremely gentle, allowing isolation of proteins as well as large or unstable complexes. The native state of proteins is preserved, and fragile cells remain viable. Flexible volumes may be used, enabling isolation of low-abundance molecules. A high signal-to noise ratio also contributes to increased sensitivity.

Easy handling – manual or automated

Magnetic separation is surprisingly easy. No tedious centrifugation, precipitation, filtration, or columns. Magnetic handling enables easy washing, separation, and concentration of your target. Excellent dispersion abilities and the lack of magnetic remanence make Dynabeads Streptavidin products ideal for manual as well as automated protocols, including microfluidic systems. Depending on your specific application and target molecule, a direct or indirect capture method is applied.

Nucleic acid purification

Library prep for NGS

  • Sequencing enrichment
  • Library generation
  • Fragmented cDNA
  • Non-invasive pre-natal testing
  • Oncology sequence enrichment of DNA

Sequence-specific capture

  • Low-abundance cDNA from libraries
  • Mutated sequences
  • RNA/DNA infectious agents
  • Microsatellite enrichment

Immobilized DNA/cDNA

  • DNA-/RNA-binding protein isolation
  • Solid-phase DNase footprinting
  • Solid-phase S1 nuclease mapping
  • Subtractive hybridization
  • Differential display
  • Limes
  • 5' RACE
  • SAGE
  • TOGA
  • RAGE

Single-stranded template

  • Solid-phase sequencing
  • Pyrosequencing
  • MALDI-MS
  • Probe generation
  • Allele-specific extension
  • In vitro mutagenesis

Protein

Protein purification

  • Intact protein complexes
  • Active enzymes
  • Immunoprecipitation
  • Protein interaction studies
  • Protein depletion
  • SDS-PAGE
  • MALDI-TOF

Immunoassays

  • Competitive /noncompetitive
  • Homogeneous /heterogeneous
  • Sandwich assays

Protein purification

  • Phage display
  • Cell-based screening
  • SELEX
  • Affibody selection
  • Drug screening

Cell

Specific cell isolation/Exosomes

  • Cell culture
  • Flow cytometry
  • Cell–cell interactions
  • Chemokine and immunological assays
  • Bacterial pathogen detection
  • Molecular analyses
  • Specific capture of exosomes

When used in combination with a biotinylated probe / ligand, any target molecule can be captured on the streptavidin-coupled Dynabeads, isolated and further manipulated.

Direct or indirect capture?

Depending on your target molecule and the specific application, the direct or indirect capture method can be applied.

For some applications, using a pre-coupled ligand for direct capture allows you to reuse the Dynabeads and thus further reduce sample preparation costs.

An indirect approach can be of benefit when the concentration of your target is low, the specific affinity is weak or the binding kinetics is slow. In the indirect approach, the probe/ligand is allowed to bind to the target in suspension prior to addition of the beads.

A monolayer of streptavidin is covalently coupled to the Dynabeads, helping to ensure negligible leakage that could otherwise disturb your assay. 

 

Four different types of Dynabeads streptavidin products are available (Table 1). Your choice should be guided by your sample and target properties, buffers and solutions applied, and specific downstream application needs.

Invitrogen Dynabeads M-280 Streptavidin and MyOne Streptavidin T1 are commonly used for protein and nucleic acid applications.

Invitrogen Dynabeads M-270 Streptavidin and MyOne Streptavidin C1 are preferred for nucleic acid protocols involving high chaotropic salt concentrations, for immunoassays involving small biotinylated antigens, and applications incompatible with BSA.

The smaller Dynabeads MyOne beads offer increased binding capacity and slower sedimentation rate, making them ideal for automated applications.

There's also a Dynabeads Streptavidin trial kit with 1 ml of each bead-type, so you can try them out with your own application.

Table 1. Dynabeads streptavidin products.

Product Capacity Characteristics and Properties Ideal for:
Dynabeads M-280 Streptavidin Free biotin:
650–900 pmoles/mg beads

Biotinylated Ig:
5–10 µg/mg beads
  • Hydrophobic bead surface
  • Based on tosylactivated beads
    Diameter: 2.8 µm
  • Size distribution: CV < 3%
  • BSA as blocking protein
    Isoelectric point: pH 5.0
  • Low charge (–10 mV (at pH 7)
  • Iron content (Ferrites): 12% (17%)
  • Immunoassays/Immunodiagnostics
  • Purification of DNA/RNA binding proteins
  • Protein purification
  • Phage display
  • Biopanning
  • Cell isolation
  • DNA enrichment and library prep e.g. for NGS
  • Sequence enrichment
Dynabeads MyOne Streptavidin T1 Free biotin:
> 1,300 pmoles/mg beads

Biotinylated Ig:
20 µg/mg beads
  • Hydrophobic bead surface
  • Based on tosylactivated beads
    Diameter: 1.05 µm
  • Size distribution: CV <3%
    BSA as blocking protein
  • Isoelectric point: pH 5.0
  • Low charge (–10 mV (at pH 7)
  • Iron content (Ferrites): 26% (37%)
  • Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
  • Immunoassays/Immunodiagnostics
  • Purification of DNA/RNA binding proteins
  • Protein purification
  • Phage display
  • Biopanning
  • Cell isolation
  • Automation
  • DNA enrichment and library prep e.g. for NGS'
  • Sequence enrichment
Dynabeads 
M-270 Streptavidin
 
Free biotin:
650–1,350 pmoles/mg beads

Biotinylated Ig:
5–10 µg/mg beads
  • Hydrophilic bead surface
  • Based on carboxylic acid beads
  • Diameter: 2.8 µm
  • Size distribution: CV <3%
  • No blocking proteins used
  • Isoelectric point: pH 4.5
  • Highly charged (–50 mV (at pH 7)
  • Iron content (Ferrites): 14% (20%)
  • Low aggregation of beads in high salt solutions
  • Sequence specific DNA/RNA capture
  • Use in protocols that require GTC lysis or high salt concentrations.
  • Preparation of single-stranded DNA
  • Immunoassays/Immunodiagnostics with hydrophobic targets
Dynabeads MyOne Streptavidin C1 Free biotin:
> 2,500 pmoles/mg beads

Biotinylated Ig:
15–20 µg/mg beads
  • Hydrophilic bead surface
  • Based on carboxylic acid beads
    Diameter: 1.05 µm
  • Size distribution: CV <3%
  • No blocking proteins used
  • Tween 20 in the buffer
  • Isoelectric point: pH 5.2
  • Medium charged (–35 mV (at pH 7))
    Iron content (Ferrites): 26% (37%)
  • Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
  • Low aggregation
  • Sequence specific DNA/RNA capture in Nucleic Acid diagnostics
  • Preparation of single-stranded DNA
  • High throughput nucleic acid clean up protocols
  • Sample preparation of proteins for mass spectrometry
  • Well suited for automated applications
Dynabeads kilobaseBINDER Kit 70 pmoles of a 4 kb biotinylated DNA fragment and 80 pmoles of a 10 kb fragment per mg beads
  • Contains Dynabeads® M-280 Streptavidin, a unique Binding Solution and a Washing Solution
  • Immobilization of long biotinylated DNA fragments (>2 kb)
  • Rapid, efficient and easy purification of nuclei from cytoplasm
  • Chromatin isolation within minutes
Dynabeads Biotin Binder Processes 2 x 109 cells (PBMC) or 200 mL whole blood
  • For use with your own standard biotinylated antobodies
  • Isolate cells from whole blood, buffy coat, PBMC, MNC or tissue digests
  • Depletion of one/multiple cell types from any sample from any species
  • Positive cell isolation for molecular downstream applications (without cell release)
CELLection Biotin Binder Kit  Processes 2 x 109 cells (PBMC) or 200 mL whole blood
  • For use with your own standard biotinylated antobodies
  • Streptavidin on the bead-surface is attached via a DNA linker, providing a cleavable site to release and emove the beads after isolation
  • Positive cell isolation and release
  • Isolate any cell from any sample from any species
Dynabeads FlowComp Flexi Processes 2 x 109 cells (MNC) or 80 mL whole blood/buffy coat
  • For use with your own antibodies
  • DSB-X biotinylation of the antibody is required to assure release of the target cells from the bead. All required ingredients are supplied in the kit
  • Positive cell isolation and release
  • Isolate any cell from any sample from any species