Distinguish single-copy differences in DNA copy number

The QuantiGene Plex DNA assay combines branched DNA (bDNA) signal amplification and multianalyte profiling beads (xMAP™) technologies to enable the detection and quantitation of multiple DNA targets simultaneously. The bDNA assay is a hybridization-based method of target-specific DNA quantitation that amplifies signal rather than target DNA.

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Unique benefits
  • Works with difficult sample types—works with degraded and cross-linked RNA in FFPE tissues and directly with blood, with no RNA purification required
  • True multiplexing—measures up to 80 genes of interest and housekeeping genes in the same well with no cross-reactivity, reduces number of samples needed
  • Standardized platform—96-well plate format compatible with Luminex 200, MagPix, and FlexMap3D™ systems
  • Simple workflow—ELISA-like workflow for direct hybridization of transcripts to beads and transcript labeling, and no reverse transcription needed
  • Large inventory of validated genes—select from over 15,000 genes to create pathway- and disease-themed panels
  • Faster customization than other methods—if we don’t have your gene(s), we can create your custom panel within 2 weeks

Video: How it works

Single copy gene resolution

QuantiGene Plex DNA Assays can distinguish as little as 20 percent differences in DNA copy number and have the ability to quantitate single copy number variations. The application below shows single-copy gene resolution and the ability to discriminate single-copy differences at the lower end of the linear range of the assay. The QuantiGene Plex DNA Assay has a 3 log linear working range and can detect up to hundreds of DNA copies.

Figure 1: Human BAC fibroblast growth factor receptor 1 (FGFR1) DNA (from 1.3 pg [0.5 copies] to 18.2 pg [7 copies]) was spiked into a constant amount of mouse genomic DNA (180 ng or 30,000 cell equivalents, based on 6 pg mouse DNA/cell). The copy number equivalents of FGFR1 were quantified using the QuantiGene DNA Assay. From 0.5 to 6 FGFR1 DNA copy equivalents per cell were easily distinguished.