Reserve your seat at this special presentation during the conference

High level of activation and altered functionality in T cell samples from subjects with progressive forms of multiple sclerosis

Speaker: Dr. Andrea Cossarizza, MD, PhD

Full professor at the University of Modena and Reggio Emilia School of Medicine, Italy

Date: Wednesday, June 14, 2016
Time: 12:45–1:45 p.m.

Abstract

Introduction: No data exists on changes in metabolic profiles of T cell subsets from individuals with multiple sclerosis after activation or differentiation, nor on their mitochondrial (mt) functionality. To improve our knowledge in this area, we examined samples from 23 individuals with various forms of the disease: 2 inactive relapsing-remitting (RR), 15 secondary progressive (SP), 6 primary progressive (PP), and 14 healthy controls (CTR).

Methods: PBMCs were stained with a large panel of mAbs to differentiate discrete cell types. Purified CD4 and CD8 T cells were in vitro stimulated with anti-CD3/CD28. Mt mass, mt O2 level, mt membrane potential (MMP), GLUT-1 receptor, and mTOR-dependent S6 ribosomal protein phosphorylation were analyzed by an Invitrogen™ Attune™ NxT Flow Cytometer.

Results: Individuals with the PP form of MS had high percentage of CD4+ TEMRA cells and low percentage of CD8+ naïve T cells vs. CTR. SP individuals displayed high percentage of CD8+ TCM cells. Both SP and PP individuals had more activated CD4+ and CD8+ T cells. T cells from individuals with all three forms of MS showed changes in S6 phosphorylation, GLUT-1 receptor expression, mt mass, O2 production, and MMP, but these changes were more marked in those with progressive MS forms.

Conclusion: Our data support the hypothesis that T cells from individuals with the PP form of MS tend to shift more rapidly and easily towards effector cells, and mediate a rapid progression of the disease.


Everyone who attends Dr. Cossarizza’s presentation will receive a custom printed plush Invitrogen blanket.*


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Speaker biography:

Dr. Andrea Cossarizza is a full professor of pathology, immunology, and clinical immunology at the University of Modena and Reggio Emilia, Italy. He has over 26 years of experience as an immunologist and has pioneered the development of new applications for flow cytometry in immunological research. As of April 2016, he has published 317 papers in peer-reviewed international journals and presented more than 90 lectures at prestigious institutions worldwide.

Dr. Cossarizza’s research is focused on the analysis of molecular and cellular alterations of the immune system in response to several different human diseases and viral infections, including HIV, hepatitis, multiple sclerosis, and Alzheimer’s disease, as well as the analysis of the changes that occur in the immune system during aging and longevity. He has pioneered novel methods for studying rare cell types, mitochondrial function in individual cells in the process of apoptosis, and the importance of mitochondrial DNA in a variety of pathologies.

Dr. Cossarizza holds several patents, including European patent (EP) 1,521,848 B1: Method of Determining the Copy Number of a Nucleotide Sequence, released January 17, 2007, which is also a US Patent (US 7,604,964 B2: Method of Determining the Copy Number of a Nucleotide Sequence, released October 20, 2009).

Specialties: Flow cytometry, systems biology, immunological techniques.