Turbulent Flow LC and MS: Research into Potential Applications for Clinical Chemistry and Therapeutic Drug Monitoring
With advances in fast chromatography techniques, and highly sensitive and selective detection methods such as mass spectrometry, high-throughput bioanalytical methods can now be easily developed. The bottleneck of the analytical process then becomes the sample preparation, which it is now realized is crucial to the robust operation of the analytical system, especially for quantitative assays. Turbulent flow liquid chromatography was developed in the late 1990s, and combines 'size exclusion' and traditional stationary phase column chemistry to separate macromolecules, such as proteins, from smaller molecules and analytes of interest in biological fluids.
In this presentation, two senior scientists from Viapath at King's College Hospital, London, will describe the theory behind Thermo Scientific™ TurboFlow™ chromatography, and illustrate application of the technology using two examples from their ongoing research; (1) the detection of catecholamine-producing tumours (pheochromocytoma and paraganglioma) by analysis of metanephrines in plasma and urine, and (2) the quantification of direct oral anticoagulants (DOACs).
For research use only. Not for use in diagnostic procedures.