PCR Amplification for Forensic DNA Profiling
AmpFℓSTR™ kits combine the sensitivity of PCR chemistry with the power of multicolor fluorescence technology to enable robust, reliable results for all human identification applications—even with mixed, degraded, and challenged samples. Many DNA casework and database laboratories have been built using AmpFℓSTR™ kits.
The AmpFℓSTR™ NGM SElect™ Express Kit enables extremely efficient analysis of single-source samples for laboratories wishing to utilize the European Standard Set Loci (ESSL) together with the highly discriminating SE33 locus.
NGM™ Kits deliver exceptional data quality and robustness along with powerful discrimination to support European cross-border data sharing initiatives. The NGM™ kit amplifies all 10 SGM Plus™ kit loci together with the 5 additional loci in the expanded European Standard Set.
NGM™ Kits deliver exceptional data quality and robustness along with powerful discrimination to support European cross-border data sharing initiatives. The NGM™ kit amplifies all 10 SGM Plus™ kit loci plus the additional loci in the expanded European Standard Set. The NGM SElect™ kit provides a parallel option that amplifies the same expanded set of loci together with the highly discriminating SE33 locus.
GlobalFiler™ STR Kits combine reduced amplification time with maximum data recovery power. The 6-dye, 24-locus technology is designed to deliver unprecedented lab performance. Both the GlobalFiler™ and GlobalFiler™ Express kits are approved for use by laboratories generating DNA profiles for inclusion in the US National DNA Index System (NDIS) CODIS database.
The Veriti™ Thermal Cycler delivers the proven reliability you expect from Applied Biosystems™ PCR instruments, plus the added control of VeriFlex™ Blocks. Veriflex™ Blocks offer you 6 independent temperature blocks providing precise control over your PCR optimization, supplying “better-than-gradient” functionality.
Optimized for casework, the AmpFℓSTR™ Identifiler™ Plus PCR Amplification Kit enables forensic analysts to recover more interpretable results from a wider range of DNA evidence samples with increased confidence.
Y-STR analysis focuses on short tandem repeats (STRs) found on the Y chromosome, only carried by male individuals. For forensic labs, the most common application of Y-STRs is in criminal cases such as sexual assault, where evidence samples are difficult to resolve with traditional autosomal STR analysis due to low amounts of male DNA present in a high background of female DNA.
Now you can simply punch a disc from a standard FTA® card and go straight to amplification. The AmpFℓSTR® Identifiler® Direct PCR Amplification Kit amplifies the 16 loci included in the Identifiler® kit from single source samples spotted on FTA® cards, without the need to perform any DNA extraction or purification. By eliminating these tedious pre-amplification steps, the Identifiler® Direct kit simplifies automation requirements and expands throughput capabilities, while maintaining high profile quality and maximizing quality assurance for the entire process.
Building on the reliability and performance of Applied Biosystems™ thermal cycler instruments, the HID Veriti™ Thermal Cycler features a 96-well (0.2 mL) format with six independent temperature zones to provide precise temperature control and rapid temperature changes for conducting PCR.
The ProFlex™ PCR System combines the reliability and performance of Applied Biosystems™ thermal cyclers with the flexible configuration and control features that fit how you work today. Dual block configurations allow you to maximize your throughput.
Dr. Antonio Alonso, a DNA expert at the National Institute of Toxicology and Forensic Sciences, Madrid (Spain), discusses the need for forensic laboratories to make the transition to 6-dye Chemistry™ technology in order to gain workflow efficiency and reduce operating costs. He highlights the value of Y-STR analysis in resolving sexual assault cases and provides the example of an 18-year-old rape/murder cold case in Spain that was solved using Applied Biosystems™ Yfiler™ Plus chemistry.
After nearly 30 years, with 54 countries and more than 70 million samples included in DNA databases worldwide, we have been successful in saving taxpayer dollars and preventing new crimes by solving crimes quickly. Tim Schellberg, JD, president of Gordon Thomas Honeywell Governmental Affairs, discusses how the global public safety community continues to advance DNA databases to their true potential. Information from the databases was used during the 2015 investigation into a Washington, DC mansion murder, where DNA from a pizza crust resulted in a DNA match, and led to the arrest of a suspect in a week.