A key challenge: DNA mixture samples from sexual assault cases contain an excess of female DNA relative to male DNA

Male autosomal component of the DNA mixture is typically too low to be detected beyond a ratio of 1:10 to 1:20 of male:female DNA. In such cases, the use of Y-STR markers allows amplification of low quantities of male DNA in a high background of female DNA.

Generate quality results with confidence

Our 6-dye chemistries of the Applied Biosystems™ QuantiFiler™ Trio, GlobalFiler™, and Yfiler™ Plus kits provide a compatible workflow to help maximize information generated on each sample, the first time through the workflow.  

Customer studies have demonstrated the ability of the QuantiFiler Trio DNA Quantification Kit to accurately predict degradation in downstream results using the GlobalFiler PCR Amplification kits. This allows laboratories to adjust input amounts to improve allele recovery. Accurate and sensitive detection of male DNA enabled by the QuantiFiler Trio allows the Yfiler Plus PCR Amplification Kit to be selected in cases of low male:female DNA ratio (M:F<1:20) where an autosomal kit would have little value.  

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Sexual assault casework workflow
1 Screen for male DNA
Analyze Male DNA

Y-screen DNA assay using
QuantiFiler
Trio kit ›

Manual Slide Search

Serological Assays

 

 
2 Extract DNA
Pipette

Differential extraction using the
Automate Express system ›

 
3 Quantify DNA

S5

Low male? Degraded DNA? Inhibited?
Low concentration?

Informative decision support on STR strategy using
QuantiFiler Trio ›

 
4 Amplify DNA

3500 Genetic Analyizer

M:F>1:20?
Use autosomal STR such as GlobalFiler kit ›

M:F<1:20?
Select Y-STR analysis using Yfiler Plus kit ›

 
5 Analyze data
Laptop with DNA

Analyze results such as quality flags using GeneMapper ID-X v1.5 Software ›

Need help bringing products online?

Look no further than our Human Identification Professional Services (HPS) team. Since 2007, the team has completed over 400 successful validation projects worldwide. 

Over 20 technical support specialists, each averaging 8 years of real-world forensic experience, provide customers with in-depth training and support on our instruments, chemistries, and software.

Learn more


Webinar and resources
Improved technology to process challenging sexual assault casework

In the first half of this webinar, Dr. Allison Holt (R&D scientist, Thermo Fisher Scientific) presents workflow improvements for analyzing challenging DNA mixture samples. During the second half, Dr. Jack Ballantyne discusses a large-scale study conducted on sexual assault samples and the ability to recover allele information using the Applied Biosystems™ QuantiFiler™ Trio and Yfiler™ Plus chemistries.
View webinar ›

A novel Y-screen application for rapid assessment of sexual assault samples

In this webinar, Dr. Allison Holt (R&D scientist, Thermo Fisher Scientific) discusses using the Quantifiler Trio kit as decision support to streamline rape kit processing, focusing on:

  • An overview of the DNA-based Y-Screen application
  • Key quality features of using the Quantifiler Trio kit in casework
  • Customer test results and a comparison of Y-screen versus traditional serology methods

View webinar ›  

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Resources

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If you have additional comments or questions, please contact the customer service team via email.

Technical support

If you are a current customer and need technical support, please contact the global service team via email, or phone us at 1-888-821-4HID (4443). Select option #1 for immediate assistance.