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Frequently Asked Questions

Answers to many of the questions you might have about ADME/Tox products, with tips and tricks for success.

What is a Rapid Alert?

Rapid Alerts notify you of the impending availability of fresh primary human hepatocytes. Note: fresh hepatocytes available in North America only. Upon receiving a Rapid Alert email, you decide if you need hepatocytes for an upcoming study and can contact us immediately to place an order. Sign up to receive our Rapid Alert.

Each Rapid Alert contains:
- a summary of the donor’s demographics, such as gender, age, race, BMI, and history of alcohol, drugs, or medications
- the plate and suspension formats available for order
- the date when the hepatocytes will be shipped to you
- a phone number and email to place your order or request additional information

How should I handle the fresh hepatocytes once I receive them?

Upon receipt, fresh hepatocytes in suspension should be centrifuged at 4ºC immediately, following our instructions. Plated hepatocytes should be quickly removed from the box and the shipping medium replaced with fresh culture medium.

The preservation medium used during shipment is meant to be kept cold; if the cells warm past 8ºC, the shipping medium begins to have a toxic effect on the hepatocytes. We recommend that you let your plated cells acclimate overnight before beginning your experiment. Check out our protocol on how to handle fresh hepatocytes in suspension

After thawing, how long can hepatocytes be kept in culture?

Unlike immortalized cell lines, hepatocytes are primary cells that cannot be cultured indefinitely. The use of thawed suspension hepatocytes should be limited to short-term experiments with a maximum of 4-6 hour incubations. 

Plateable hepatocytes, which attach to collagen-coated plasticware in culture media, are generally metabolically active for 5-7 days.

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Find the latest technical information for your ADME/Tox research applications. 

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ADME/Tox Product Brochure

Rat Kupffer Cryopreserved Co-cultures

HEP10 Pooled Human Cryopreserved Hepatocytes 

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International Society for the Study of Xenobiotics - October 18-22, 2015, Orlando, FL – Register now


Thawing and Plating Cryopreserved Hepatocytes
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