Aromatase, a product of the CYP19A1 gene, is a member of the cytochrome P450 superfamily of monooxygenases that catalyze numerous reactions involved in drug metabolism and the synthesis of steroids, cholesterol, and other lipids. Aromatase is localized on the endoplasmic reticulum and catalyzes the formation of aromatic C18 estrogens from C19 androgens.
Aromatase is found in many tissues including the adrenal glands, gonads, placenta, skin, and adipose, as well as a number of abnormal tissues including those associated with endometriosis, uterine fibroids, breast cancer, and endometrial cancer. A surplus of aromatase leads to aromatase excess syndrome (AES), in which excess levels of estrogen result in heterosexual precocity in males and isosexual precocity in females. Furthermore, a lack of aromatase leads to aromatase deficiency (AROD), a rare disease in which fetal androgens are not converted into estrogens because of placental aromatase deficiency. This deficiency causes the virilization of female fetuses and primary amenorrhea, polycystic ovaries, breast hypoplasia, and tall stature in adult females.
Aromatase antibodies are commonly used in research applications such as western blot, immunohistochemistry, and immunofluorescence. Quality Invitrogen aromatase receptor antibodies are available for a variety of research needs.
Featured product data
Immunohistochemical analysis of aromatase using a specific polyclonal antibody (Cat. No. PA1-21398).
Western blot analysis using an aromatase polyclonal antibody (Cat. No. PA5-14517) in Jurkat cell lysates (35 µg per lane).
Immunofluorescent staining of HEPG2 cells using a specific aromatase antibody (Cat. No. PA5-19633). The cells were fixed with formaldehyde (4%) for 10 minutes, permabilized with BSA (1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% T-BST for 1 hour and exposed to the primary antibody at a concentration of 5 µg/mL overnight at 4°C. The secondary antibody was a FITC-conjugated goat anti-rabbit IgG (green) at a dilution of 1:1000. Plasma membranes were labeled with a WGA-594 fluorescent conjugate (red), and the nuclei stain was DAPI (blue).
For Research Use Only. Not for use in diagnostic procedures.