Beta galactosidase (beta-Gal or β-Gal) is an enzyme that catalyzes the hydrolysis of beta galactosides into monosaccharides and is an essential enzyme in the human body. Deficiencies in the beta galactosidase protein can result in galactosialidosis or Morquio B syndrome.
In E. coli, the lacZ gene, which codes for beta galactosidase, is part of the lac operon that is activated in the presence of lactose when the glucose level is low. Beta galactosidase is widely used as a reporter marker to monitor gene expression. It is also used in the blue/white screen for detecting recombinant bacteria. In addition, beta galactosidase is highly expressed in the lysosomes of senescent cells, so it is frequently used as a biomarker to detect aging and senescent cells. Quality Invitrogen beta galactosidase antibodies are available for a variety of research needs.
Featured product data
Immunofluorescent analysis of E. coli beta-galactosidase (green) in pCMV-LacZ transfected HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Cat. No. 37525) for 15 minutes at room temperature. Cells were probed with a beta-galactosidase monoclonal antibody (Cat. No. MA1-152) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Cat. No. 35502). Nuclei (blue) were stained with Hoechst 33342 dye (Cat. No. 62249). Images were taken on a Thermo Scientific ToxInsight instrument at 20x magnification.
Cell analysis resources
- Cell Imaging Reagent Guide & Protocol App
- Fluorescence and Flow Cytometry Tutorials
- Antibodies for Flow Cytometry
- Antibodies for Western Blotting
- The Molecular Probes Handbook
- Cell Analysis Protocols
- The Cell Culture Basics Handbook
- Cell Analysis Technical Resources
- Labeling Chemistry Selection Tool
- Newsletters & Journals
For Research Use Only. Not for use in diagnostic procedures.