The HA (hemagglutinin) tag is derived from the human influenza virus HA protein. It has been extensively used as a general antibody epitope tag and is well-characterized. HA tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe.

Invitrogen HA tag antibodies reliably detect recombinant HA tagged proteins, and each antibody is validated for use in a variety of applications.

See all anti-HA tag antibodies

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Western blot analysis of HA epitope tag. Analysis was performed by running various amounts of E. coli lysate containing a multi-epitope tagged protein per well in a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HA epitope tag monoclonal antibody (Cat. No. 26183) at a dilution of 1:10,000 for 1 hour at room temperature on a rocking platform, washed in TBS-0.1%Tween™ 20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Cat. No. 32430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using Thermo Scientific SuperSignal West Pico Chemiluminescent Substrate (Cat. No. 34080).

Immunofluorescent analysis of HeLa cells transfected with a construct containing an HA epitope tag. Formalin fixed cells were permeabilized with 0.1% Triton™ X-100 in TBS for 10 minutes at room temperature and blocked with 1% Thermo Scientific Blocker BSA (Cat. No. 37525) for 15 minutes at room temperature. Cells were probed with a DyLight 488-conjugated HA epitope tag monoclonal antibody (Cat. No. 26183-D488) at a dilution of 1:25 for at least 1 hour at room temperature. Nuclei (blue) were stained with Hoechst™ 33342 dye (Cat. No. 62249). Images were taken on a Thermo Scientific ArrayScan imager at 20x magnification.

Annotated product references

  • Cat. No. 26183 was used in immunoprecipitation to identify novel protein co-factors of the beta2 isoform of the thyroid hormone receptor. Molecular endocrinology (Baltimore, Md.) (May 2013; 27: 840) "Research resource: identification of novel coregulators specific for thyroid hormone receptor-β2." Hahm JB,Privalsky ML
  • Cat. No. 26183 was used in immunoprecipitation to evaluate a recombinant myeloperoxidase antibody. Analytical biochemistry (Mar 2011; 410: 1) "A high-affinity recombinant antibody permits rapid and sensitive direct detection of myeloperoxidase." McDonnell B,Hearty S,Finlay WJ,O'Kennedy R
  • Cat. No. PA1-985 was used in western blot to study the roles of PAN1 and PAN2 in the polarization of maize cell division. The Plant cell ( 2012; 24: 4577) "Identification of PAN2 by quantitative proteomics as a leucine-rich repeat-receptor-like kinase acting upstream of PAN1 to polarize cell division in maize." Zhang X,Facette M,Humphries JA,Shen Z,Park Y,Sutimantanapi D,Sylvester AW,Briggs SP,Smith LG

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