Protein Conjugates for neuron staining

This class of neuron stains comprises fluorescent conjugates of cholera toxin subunit B, wheat germ agglutinin (WGA), and isolectin GS-IB4. Introduced via microinjection or iontophoresis, these probes are fixable and photostable retrograde tracers and offer choices for synaptic studies (WGA) and to differentiate between neuronal subtypes (GS-IB4).


Select protein conjugates for neuron staining

Protein Conjugates for Neuron Staining

  • Exogenous staining
  • Fixable and photostable
  • Choice of retrograde tracers
  • WGA crosses synapses

Cholera toxin subunit B binds to glycosphingolipids in axonal membranes and serves as a retrograde tracer. Wheat germ agglutinin (WGA) conjugates bind to sialic acid and N-acetylglucosaminyl residues in neuronal membranes and serve as retrograde tracers that will also cross synapses. Isolectin GS-IB4 is selective for terminal α-D-galactosyl residues and serves to differentiate between neuronal subtypes.

  Protein Conjugates
Human cheek epithelial cells labeled with Alexa Fluor® 350 wheat germ agglutinin and stained with SYTOX® Green nucleic acid stain.

Selection Guides for Protein Conjugates

 
Readout Direct fluorescence imaging of cells with punctate signal
Carrier Wheat Germ Agglutinin
Standard filter set(s) Cy®5
Fluorophore/conjugate Qdot® 655
Ex/Em (nm) /655
Tissue sections Yes
In vivo neurons No
Fixed tissue sections No
Cultured cells Yes
Cell loading Iontophoresis
Staining mode Exogenous
Best for Cell populations
Fixable Yes
Transport Retrograde
Photobleaching resistance 5 stars
Synaptic transfer Yes
Gap junction transfer No
Bibliography Citations
Quantity 200 µL
Cat. No. Q12021MP

Properites

Readout Direct fluorescence imaging of cells with uniform signal distribution, binds to sialic acid and N-acetylglucosaminyl residues in neuronal membranes
Carrier Wheat Germ Agglutinin
Tissue sections Yes
In vivo neurons No
Fixed tissue sections No
Cultured cells Yes
Cell loading Iontophoresis
Staining mode Exogenous
Best for Cell populations
Fixable Yes
Transport Retrograde
Photobleaching resistance 5 stars
Synaptic transfer Yes
Gap junction transfer No
Bibliography Citations

 

Selection Guide

 
Standard filter set(s)
Fluorophore/
conjugate
Ex/Em (nm)
Quantity
Cat. No.
Wheat Germ Agglutinin, Alexa Fluor® 350 Conjugate DAPI Alexa Fluor® 350 346/442 5 mg W11263
Wheat Germ Agglutinin, Alexa Fluor® 488 Conjugate FITC Alexa Fluor® 488 495/519 5 mg W11261
Wheat Germ Agglutinin, Alexa Fluor® 555 Conjugate TRITC Alexa Fluor® 555 555/565 5 mg W32464
Wheat Germ Agglutinin, Alexa Fluor® 594 Conjugate Texas Red® Alexa Fluor® 594 590/617 5 mg W11262
Wheat Germ Agglutinin, Alexa Fluor® 633 Conjugate Texas Red® Alexa Fluor® 633 632/647 5 mg W21404
Wheat Germ Agglutinin, Alexa Fluor® 647 Conjugate Cy®5 Alexa Fluor® 647 650/668 5 mg W32466
Wheat Germ Agglutinin, Alexa Fluor® 680 Conjugate Cy®5.5 Alexa Fluor® 680 679/702 5 mg W32465
 
Readout Binds to ganglioside GM1 to label lipid rafts; requires secondary detection Direct fluorescence imaging of cells, selective for ganglioside GM1
Carrier Cholera Toxin Subunit B (Recombinant)
Standard filter set(s) NA FITC TRITC Texas Red® Cy®5
Fluorophore/conjugate Biotin-XX Horseradish peroxidase Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647
Ex/Em (nm) NA 495/519 555/565 590/617 650/668
Tissue sections Yes
In vivo neurons No
Fixed tissue sections No
Cultured cells Yes
Cell loading Iontophoresis, microinjection
Staining mode Exogenous
Best for Cell populations
Fixable Yes
Transport Retrograde
Photobleaching resistance NA 5 stars
Synaptic transfer No
Gap junction transfer No
Bibliography Citations
Quantity 100 µg 100 µg 100 µg 100 µg 100 µg 100 µg
Cat. No. C34779 C34780 C34775 C34776 C34777 C34778
 
Readout Selective for terminal α-D-galactosyl residues; requires secondary detection Direct fluorescence imaging of cells, selective for terminal α-D-galactosyl residues
Carrier Isolectin GS-IB4 from Griffonia simplicifolia
Standard filter set(s) NA FITC Rhodamine Texas Red® Cy®5
Fluorophore/conjugate Biotin-XX Alexa Fluor® 488 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647
Ex/Em (nm) NA 495/519 578/603 590/617 650/668
Tissue sections Yes
In vivo neurons Yes
Fixed tissue sections No
Cultured cells Yes
Cell loading Microinjection, iontophoresis
Staining mode Exogenous
Best for Cell populations
Fixable Yes
Transport Bidirectional
Photobleaching resistance NA 5 stars
Synaptic transfer No
Gap junction transfer No
Bibliography Citations
Quantity 500 µg 500 µg 500 µg 500 µg 5 mg
Cat. No. I21414 I21411 I21412 I21413 W32466
 
Readout Direct fluorescence imaging of cells with punctate signal
Carrier Wheat Germ Agglutinin
Standard filter set(s) Cy®5
Fluorophore/conjugate Qdot® 655
Ex/Em (nm) /655
Tissue sections Yes
In vivo neurons No
Fixed tissue sections No
Cultured cells Yes
Cell loading Iontophoresis
Staining mode Exogenous
Best for Cell populations
Fixable Yes
Transport Retrograde
Photobleaching resistance 5 stars
Synaptic transfer Yes
Gap junction transfer No
Bibliography Citations
Quantity 200 µL
Cat. No. Q12021MP

Properites

Readout Direct fluorescence imaging of cells with uniform signal distribution, binds to sialic acid and N-acetylglucosaminyl residues in neuronal membranes
Carrier Wheat Germ Agglutinin
Tissue sections Yes
In vivo neurons No
Fixed tissue sections No
Cultured cells Yes
Cell loading Iontophoresis
Staining mode Exogenous
Best for Cell populations
Fixable Yes
Transport Retrograde
Photobleaching resistance 5 stars
Synaptic transfer Yes
Gap junction transfer No
Bibliography Citations

 

Selection Guide

 
Standard filter set(s)
Fluorophore/
conjugate
Ex/Em (nm)
Quantity
Cat. No.
Wheat Germ Agglutinin, Alexa Fluor® 350 Conjugate DAPI Alexa Fluor® 350 346/442 5 mg W11263
Wheat Germ Agglutinin, Alexa Fluor® 488 Conjugate FITC Alexa Fluor® 488 495/519 5 mg W11261
Wheat Germ Agglutinin, Alexa Fluor® 555 Conjugate TRITC Alexa Fluor® 555 555/565 5 mg W32464
Wheat Germ Agglutinin, Alexa Fluor® 594 Conjugate Texas Red® Alexa Fluor® 594 590/617 5 mg W11262
Wheat Germ Agglutinin, Alexa Fluor® 633 Conjugate Texas Red® Alexa Fluor® 633 632/647 5 mg W21404
Wheat Germ Agglutinin, Alexa Fluor® 647 Conjugate Cy®5 Alexa Fluor® 647 650/668 5 mg W32466
Wheat Germ Agglutinin, Alexa Fluor® 680 Conjugate Cy®5.5 Alexa Fluor® 680 679/702 5 mg W32465
 
Readout Binds to ganglioside GM1 to label lipid rafts; requires secondary detection Direct fluorescence imaging of cells, selective for ganglioside GM1
Carrier Cholera Toxin Subunit B (Recombinant)
Standard filter set(s) NA FITC TRITC Texas Red® Cy®5
Fluorophore/conjugate Biotin-XX Horseradish peroxidase Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647
Ex/Em (nm) NA 495/519 555/565 590/617 650/668
Tissue sections Yes
In vivo neurons No
Fixed tissue sections No
Cultured cells Yes
Cell loading Iontophoresis, microinjection
Staining mode Exogenous
Best for Cell populations
Fixable Yes
Transport Retrograde
Photobleaching resistance NA 5 stars
Synaptic transfer No
Gap junction transfer No
Bibliography Citations
Quantity 100 µg 100 µg 100 µg 100 µg 100 µg 100 µg
Cat. No. C34779 C34780 C34775 C34776 C34777 C34778
 
Readout Selective for terminal α-D-galactosyl residues; requires secondary detection Direct fluorescence imaging of cells, selective for terminal α-D-galactosyl residues
Carrier Isolectin GS-IB4 from Griffonia simplicifolia
Standard filter set(s) NA FITC Rhodamine Texas Red® Cy®5
Fluorophore/conjugate Biotin-XX Alexa Fluor® 488 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647
Ex/Em (nm) NA 495/519 578/603 590/617 650/668
Tissue sections Yes
In vivo neurons Yes
Fixed tissue sections No
Cultured cells Yes
Cell loading Microinjection, iontophoresis
Staining mode Exogenous
Best for Cell populations
Fixable Yes
Transport Bidirectional
Photobleaching resistance NA 5 stars
Synaptic transfer No
Gap junction transfer No
Bibliography Citations
Quantity 500 µg 500 µg 500 µg 500 µg 5 mg
Cat. No. I21414 I21411 I21412 I21413 W32466