HCS image showing assay features as an overlay

Assay protocols and automated analysis

High-content screening (HCS) or high-content analysis (HCA), reports quantitatively on parameters such as individual cell or organelle morphology and spatial distribution of targets. Invitrogen fluorescent reagents are the preferred tools for labeling and detection of cellular targets. Thermo Scientific HCS Studio Cell Analysis Software is used with images from EVOS FL Auto 2 or Thermo Scientific HCA platforms to help you combine those parameters and build applications that answer complex biological questions.

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Applications for EVOS FL Auto 2 and Thermo Scientific HCA platforms



Cell viability

Mitochondrial health

Protein synthesis

Transcription factor activation


Cell cycle and mitotic index

Cytoskeletal rearrangement

Neurite outgrowth

Stem cell differentiation


Cell proliferation


Oxidative stress



Instrument systems

HCS reagents optimized for use with your HCS platform

HCS application notes