Technical and educational videos for flow cytometry topics

These videos, tutorials and webinars are designed to provide technical and education information regarding flow cytometers as well as flow cytometry research applications and techniques.


Featured videos

How recent technological advances in flow cytometry instrumentation are enabling faster throughput

This webinar presented by J. Paul Robinson from Purdue University, focuses on advances in the Invitrogen Attune NxT Flow Cytometer and outlines where the technology within the Attune NxT system fits in current and future applications.

CYToMORE 2016—Advances in Cytometry

The 6th Modena Advanced Course in Cytometry (CYToMORE), was aimed at researchers who wanted to improve their flow cytometry knowledge and learn new applications. Organized in collaboration with the Italian Society of Immunology, Clinical Immunology and Allergology (SIICA) and led by teachers who are among the top international experts in the field, 2016 was a huge success. In this presentation, Victor Appay, Faculté de Médecine Pierre et Marie Curie, at Pitié-Salpêtrière, France discusses his work on “Assessing immunological aging using flow cytometry”.


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Type Title and description Categories
CYToMORE 2016 Lecture ...and on the 8th day, God created cytometry
Presented by J. Paul Robinson, Purdue University, USA
flow cytometer/flow cytometry
CYToMORE 2016 Lecture Assessing immunological aging using flow cytometry
Presented by Victor Appay, Faculté de Médecine Pierre et Marie Curie, Pitié-Salpêtrière, France
flow cytometer/flow cytometry, immunophenotyping
CYToMORE 2016 Lecture Asymptomatic but threatening: dissecting the consequences of cytomegalovirus infection
Presented by Anis Larbi, Singapore Immunology Network – SigN, Singapore
flow cytometer/flow cytometry
CYToMORE 2016 Lecture Cancer stem cells and multi-drug resistance by flow cytometry
Presented by Jordi Petriz, Josep Carreras Leukaemia Research Institute, IJC, Spain
cancer, flow cytometer/flow cytometry, multidrug resistance (MDR), stem cell research
CYToMORE 2016 Lecture Cytometric analysis of cytokines and transcription factors
Presented by Hyun-Dong Chang, Deutsches Rheuma-Forschungszentrum, Berlin, Germany
cell signaling, flow cytometer/flow cytometry
CYToMORE 2016 Lecture Cytometry of immunity
Presented by Andreas Radbruch, Deutsches Rheuma-Forschungszentrum Berlin, Germany
flow cytometer/flow cytometry
CYToMORE 2016 Lecture Cytomic analysis of oxidative stress
Presented by J. Enrique O’Connor, University of Valencia, Spain
flow cytometer/flow cytometry, oxidative stress
CYToMORE 2016 Lecture High throughput and high content flow cytometry
Presented by J. Paul Robinson, Purdue University, USA
Attune/Attune NxT, flow cytometer/flow cytometry, high content analysis, high throughput screening
CYToMORE 2016 Lecture Heterogeneity of tumour-infiltrating leukocytes: implications for adoptive cell transfer immunotherapy
Presented by Enrico Lugli, Humanitas Clinical and Research Center, Italy
flow cytometer/flow cytometry
CYToMORE 2016 Lecture Host-microbe interaction at mucosal surfaces
Presented by Maria Rescigno, Istituto Europeo di Oncologia – IEO, Italy
flow cytometer/flow cytometry, microbiology
CYToMORE 2016 Lecture Mass cytometry in the phenomics era: an essential tool for gene/function studies
Presented by Herve Luche, Centre d’Immunophénomique - CIPHE, France
flow cytometer/flow cytometry, mass cytometry
CYToMORE 2016 Lecture Microenvironment cytometry
Presented by Paul Smith, Cardiff University, UK
flow cytometer/flow cytometry
CYToMORE 2016 Lecture Novel reagent solutions for flow cytometry
Presented by Jolene Bradford, Thermo Fisher Scientific, USA
Attune/Attune NxT, flow cytometer/flow cytometry
CYToMORE 2016 Lecture Rare cells analysis: from bench to clinics
Presented by Andrea Cossarizza, University of Modena and Reggio Emilia, Italy
Attune/Attune NxT, flow cytometer/flow cytometry
CYToMORE 2016 Lecture RNA flow cytometry
Presented by Paul Wallace, Roswell Park Cancer Institute, USA
flow cytometer/flow cytometry, RNA
CYToMORE 2016 Lecture Software tutorial: what’s new
Presented by Carmen Gondhalekar, Purdue University, USA
Attune/Attune NxT, flow cytometer/flow cytometry
CYToMORE 2016 Lecture Tuning acoustic forces for flow cytometry
Presented by Jolene Bradford, Thermo Fisher Scientific, USA
Attune/Attune NxT, flow cytometer/flow cytometry
eLearning Course T cell stimulation and proliferation
This modular, animated, and narrated eLearning course was developed to provide a succinct, contextual summary of T cell activation and the methods used to measure T cell function. There are knowledge checks throughout the course as well as a practical application session to test what you have learned. Registration on LabRoots.com is required to access this course.
Attune/Attune NxT, ELISA, flow cytometer/flow cytometry, immunophenotyping, multiplex ELISA/Luminex, T cell differentiation, T cell proliferation
Tutorial Tutorial: Labeling a purified antibody
Educational video on how to label your next antibody for imaging or fluorescence. Walk through the tutorial as Molecular Probes scientists demonstrate the protocol—including all the tips and tricks you'll want to know about for your next antibody labeling experiment. The video features Judie showing Curtis, a chemistry graduate student, how to label his monoclonal antibodies while saving time and maximizing yield.
antibodies, antibody labeling, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Tutorial Molecular Probes Tutorial Series—Analyzing Flow Cytometry Data flow cytometer calibration, flow cytometer/low cytometry, multicolor flow cytometry
Tutorial Molecular Probes Tutorial Series—Anatomy of Fluorescence Spectra absorbance, emission, excitation, spectra
Tutorial Molecular Probes Tutorial Series—Introduction to Flow Cytometry flow cytometer calibration, flow cytometer/flow cytometry, multicolor flow cytometry
Tutorial Molecular Probes Tutorial Series—Introduction to Fluorescence fluorescence
Tutorial Molecular Probes Tutorial Series—Overview of Filters and Light Sources emission, excitation, filters, light cubes, light sources
Video Click-iT Plus EdU Proliferation Assay
Measuring DNA synthesis is the most precise way to detect changes in cell proliferation. Image-based proliferation assays generate spatial and temporal results that can not be detected with other methods. This video describes and compares two of the most referenced image-based proliferation technologies.
cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging
Video How to site specifically label your antibody using SiteClick technology
This video explains how to easily and site specifically label an antibody using an enzymatic and Click chemistry approach. This method can be applied to any intact IgG antibody and requires no antibody engineering or complex methodology. Unlike classic antibody conjugation techniques this breakthrough SitClick technology allows for antibody conjugation with complete confidence that the label will not directly interfere with the antibody binding domain.
antibodies, antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, Qdot, western detection
Webinar A practical approach to antibody labeling
The growing number of fluorophores available makes labeling your own antibodies a tempting proposition. But with many antibody labeling solutions available, selecting the best option can be a daunting task. In this webinar we will:
  • Provide an overview of our antibody labeling kits
  • Offer guidance on which methods are ideal for specific applications and experiments
  • Provide tips and tricks to optimize your labeling protocol
antibodies, antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF)
Webinar An introduction to flow cytometric analysis, Part 1: Cell proliferation analysis
In this 2-part series, we will give an overview of tools and techniques using Invitrogen reagents for flow cytometric analysis of cell proliferation, viability, vitality, and apoptosis.
apoptosis, cell cycle, cell proliferation, flow cytometer/flow cytometry, multicolor flow cytometry, viability
Webinar An introduction to flow cytometric analysis, Part 2: Cell viability and apoptosis analysis
In this free webinar, we will discuss flow cytometric analysis of apoptosis and identification of dead cells. Changes introduced by apoptosis can be tested with numerous assays measuring Membrane structure, Mitochondrial function, Metabolism, Caspase activity, Membrane integrity, and DNA fragmentation. We will also discuss dead cell identification using traditional impermeant nucleic acid dyes.
apoptosis, cell cycle, cell proliferation, flow cytometer/flow cytometry, multicolor flow cytometry, viability
Webinar Basics of flow cytometry, Part I: Gating and data analysis
An introduction to fluorescence, this presentation covers the following topics: the principle of the flow cytometry platform, the basic components of a flow cytometer, how to interpret a dye excitation/emission spectrum, how data is displayed, basic gating demonstration, and common statistics and terminology used in flow cytometry.
flow cytometer calibration, flow cytometer/flow cytometry, flow cytometry data, flow cytometry set up, multicolor flow cytometry
Webinar Basics of flow cytometry, Part II: Compensation
This presentation provides an overview of basic fluorochromes used in flow cytometry. Topic includes: the principle of compensation, how to perform compensation, the types of controls recommended and their use, basic strategies for designing a flow experiment, and data presentation.
flow cytometer calibration, flow cytometer/flow cytometry, flow cytometry data, flow cytometry set up, multicolor flow cytometry
Webinar Basics of multicolorflow cytometry panel design
With the proliferation of new fluorescent dyes, as well as instruments that can detect 18 or more parameters multicolor flow cytometry has become more popular and more accessible than ever. This webinar presented by Dr. Holden T. Maecker at Stanford University will discuss the caveats of good panel design, including:
  • Rules for designing panels
  • Examples and practical application of these rules
  • Controls and standardization
  • Relevance of panel design to new mass cytometry platforms
antibodies, compensation, flow cytometer/flow cytometry, multicolor flow cytometry, viability
Webinar DNA content cell cycle analysis using flow cytometry
Find out how careful acquisition and methodical preparation contribute to accurate and consistent DNA analysis. In this webinar we’ll discuss:
  • An overview of the methods and materials for using flow cytometry to determine cell cycle by measuring DNA content
  • Selection of DNA dyes for live cell and fixed cell analysis
  • Tips and tricks for consistent results
cell counting, cell cycle, cell proliferation, flow cytometer/flow cytometry
Webinar Flow cytometry in microbiological research
In recent years the application of flow cytometry in microbiological research has expanded from detection and quantification of organisms to more complex studies including analysis of host-microbe interactions and detailed spatial and temporal analysis of microbial metabolism in different environments. During this webinar we will discuss how the multi-parametric nature of flow cytometry can be applied to microbiology and the advantages of using this application over traditional microbiological methods.
flow cytometer/flow cytometry, microbiology, multicolor flow cytometry, viability
Webinar How recent technological advances in flow cytometry instrumentation are enabling faster throughput
This webinar presented by J. Paul Robinson at Purdue University, will focus on advances in the Invitrogen Attune NxT Flow Cytometer and outline where the technology within the Attune NxT fits in current and future applications.
Attune/Attune NxT, cell cycle, flow cytometer/flow cytometry, multicolor flow cytometry
Webinar Protein and Cell Analysis Upcoming Educational Webinars
See our upcoming webinars and see what is now available on demand in our virtual learning platform.
Attune/Attune NxT, flow cytometer/flow cytometry
Webinar Learn to choose the right fluorophore when designing experiments
The choice of fluorophore is one of the first important decisions to make in developing an experiment. Fluorophores are compounds that emit light at a specific wavelength when they have been excited at another, lower wavelength. Join our webinar and explore:
  • How to choose the best organic dye for an assay
  • Quantum dots and how they compare to other dyes
  • When to use a phycobiliprotein like R-PE or APC
  • When to use fluorescent proteins like GFP
  • How to choose a suitable dye to match your instrument
In addition, we will explore the basic characteristics, strengths, and weaknesses of the various fluorophores to help you choose and develop the best assay for your needs.
antibodies, antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins