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Molecular Probes® Antibody Labeling Kits label 100 µg IgG polyclonal and monoclonal antibodies with our superior Alexa Fluor®, Pacific Blue™, and Pacific Orange™ dyes.

  • Simple–labeled antibodies ready in 90 minutes requiring approximately 15 minutes actual hands-on time.
  • Reliable–protein conjugates with stable attachment of fluorescent labels for subsequent use in fluorescence-based imaging or flow cytometry.
  • Convenient–eliminates background staining commonly observed with secondary antibodies and allows more than one same-species antibody in a single experiment.

Antibody Labeling Kit Products

Fluorophore Cat. no.
Emission Color*
Flow Cytometry Imaging
Alexa Fluor® 350 A20180           ●
Alexa Fluor® 488 A20181            ●         ●
Alexa Fluor® 532 A20182           ●
Alexa Fluor® 546 A20183           ●
Alexa Fluor® 555 A20187           ●
Alexa Fluor® 568 A20184           ●
Alexa Fluor® 594 A20185           ●
Alexa Fluor® 647 A20186            ●         ●
Alexa Fluor® 680 A20188            ●         ●
Alexa Fluor® 790 A20189           ●
Pacific Blue™ dye P30013            ●         ●
Pacific Orange™ dye P30014            ●  
* Gray represents fluorescence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm.

How Antibody Labeling Kits Work

The Antibody Labeling Kits utilize an amine-reactive Alexa Fluor®, Pacific Blue™ or Pacific Orange™ fluorophore to covalently attach the label to the IgG antibody of interest.  Once formed, the covalent bond between the label and the protein is extremely stable and you are using the same chemistry we use to prepare our own primary and secondary conjugates.

These kits are optimized for labeling 100 µg of antibody per reaction. Comparably small amounts of other proteins (>40 kDa) can also be labeled. Simply adjust the protein concentration to ~1 mg/ml in the provided buffer, then add it to a vial of amine-reactive dye. Any stabilizing proteins (i.e., BSA) must be removed prior to the labeling reaction.

Purification is accomplished on a size exclusion spin column optimized for ≥40 kDa proteins (Figure 1). The entire labeling and purification procedure can be completed in as little as 90 minutes and everything needed to perform five conjugations is provided.

APEX Antibody Labeling Kits


Figure 1. Simply adjust the protein concentration to ~1 mg/ml in the provided buffer, then add it to a vial of amine-reactive dye. Purification is accomplished on a size exclusion spin column optimized for ≥40 kDa proteins.

 
Each Antibody Labeling Kit includes:
Material Amount
Reactive fluorescent label 5 vials
Sodium bicarbonate 1 vial
purification resin 1 vial
Spin columns 5
Collection tubes 5


Product Information Sheet

Need to Label Other Antibody Amounts?

Zenon® Kits

< 1-20 µg
Microscale Kits

< 1-20 µg
Antibody Labeling kits

< 1-20 µg



 

Protein Labeling Kits

< 1-20 µg

 

SAIVI™ Kits

< 1-20 µg

 

APEX™ Antibody Labeling kits

10-20 µg




Learn More about APEX™ Antibody Labeling Kits