iPSCs have revolutionized the field of stem cell research, serving as models to help define mechanisms of disease pathology and playing a vital role in drug discovery and identification of therapeutic targets. This handbook serves as a resource for the pluripotent stem cell workflow and provides recommendations for the use of related tools.
Cell Culture Information
Controlled environments, careful handling
Cell culture is viewed as one of those enigmatic techqniques by some researchers. There are so many parameters to consider: which cells to use, what media and supplements are appropriate, how often to feed, how to deal with contamination, etc. We've put together a collection of resources that we hope will give you the confidence to get started and to continuously improve your skills in the cell culture room.
Cell culture features
Virtual lab training
The Gibco Education virtual labs represent a unique interactive cell culture training experience. Learn everything from the fundamentals of cell culture to key techniques for primary and stem cell cultures and successful protein expression. As you progress through each lab, you can certify your knowledge by taking in-lab quizzes.
Intended as an introduction to cell culture, the handbook covers topics such as getting familiar with the requirements of a laboratory dedicated to cell culture experiments, laboratory safety, aseptic technique, and microbial contamination of cell cultures, as well as providing basic methods for passaging, freezing, and thawing cultured cells.
Having the right components for your complete neural cell culture medium is essential to achieving the best cell culture results. Find the optimal basal medium and supplement for primary neurons, glial cells, and astrocytes using this guide.
No matter how carefully you handle your cultures once your work is underway, it won’t prevent misidentified or corrupted cultures from coming through the doors. The best way for researchers to protect their work—and by extension their reputations—is through consistent authentication practices.
Whether you’re culturing individual cell lines or scaling up for high throughput screening, or anything in between, there’s a Thermo Scientific™ Nunc™ dish, multi-well plate, or microplate for your needs. Advances in manufacturing for surface technology, well geometry and optical flatness mean we have a plate tailored for your specific application.
The Nunc™ UpCell Surface enables harvesting of cells with high viability and intact surface proteins for culture passaging, single-cell analyses, and cell transplantation research. This application note outlines the advantages of cell harvesting without trypsin or scraping and shows results for three different use cases.
Culturing embryonic stem cells using media filtered with Thermo Scientific Nalgene Rapid-Flow PES filter units to reduce contamination risk
Researchers commonly forgo media filtration when preparing media for embryonic stem cell (ESC) culture out of fear of removing critical media components or adding deleterious compounds during the filtration process, but this can lead to an increased risk of contamination. This application note demonstrates that ESCs cultured using media filtered through Rapid-Flow PES filters maintain normal growth and pluripotency.