ExpiSf CD Medium
Experience the highest consistency and protein expression with a chemically defined formulation.

ExpiSf CD Medium is a GMP-manufactured, chemically defined, yeastolate-free, serum-free, protein-free, animal origin-free medium specifically designed for high-density growth, transfection, and baculovirus infection of suspension-adapted Sf9 (Spodoptera frugiperda) insect cells.

Delivers high reproducibility and lot-to-lot reliability.

Facilitates high-level expression of baculovirus-infected cultures, achieving protein yields three times higher than traditional insect expression platforms when used with other components of the ExpiSf Expression System.

Sf-900 III SFM
An animal origin-free formulation to support high density growth and protein expression

Optimized to support Sf9 and Sf21 cells—achieves Sf9 densities of 10-14 X 106 cells/ml
Sf9 and Sf21 cells pre-adapted to Sf-900 SFM available providing faster turnaround.

Contains lower hydrolysate concentration compared to other commercially available serum free media
Animal origin-free — facilitates regulatory processes.

Sf-900 II SFM
A protein-free medium optimized to support and maximize Sf9 and Sf21 cell growth in suspension applications

Optimized for recombinant gene expression in both baculovirus and stable insect expression systems

Express Five SFM
A protein-free medium optimized to support maximum cell growth

Optimized for recombinant gene expression by the baculovirus expression by the baculovirus expression vector system (BEVS) in the BTI-TN-5B1-4 (High Five) Trichoplusia ni cell line.

Table 1. Gibco insect cell culture media.

Product Optimized for Protein free Serum free Animal origin free Preadapted cell lines available
ExpiSf CD Medium

Suspension ExpiSf9, Sf9, Sf21 cells 
(Spodoptera frugiperda)

X X X ExpiSF9 Cells (A35243)
Sf-900 III SFM

Suspension Sf9, Sf21 cells 
(Spodoptera frugiperda)

X X   Sf9 Cells
(12659-017)
Sf21 Cells
(12682-019)
Sf-900 II SFM* Suspension Sf9, Sf21 cells
(Spodoptera frugiperda), TN368 cells (Trichoplusia ni)
X X   Sf9 Cells
(11496-015)
Sf21 Cells
(11497-013)
Express Five  SFM*
Suspension BT1-TN-5B1-4 insect cells X X   High Five™ Cells
(B855-02)

All four media used for the growth and maintenance of cells in the baculovirus expression vector systems (BEVS) for adherent or suspension culture. Also for large-scale production of recombinant proteins expressed by BEVS.

*Drug Master File Available.
Note: Cell lines from different sources, and different clones of the same cell line, may have highly specific nutritional requirements and may therefore prefer one medium over another. More than one medium formulation (if available) should be evaluated to determine the best option.

Serum Free Media Adapted Cells